 | Figure 1
Nature Biotechnology
20, 1035 - 1039 (2002)
Published online: 23 September 2002; | doi:10.1038/nbt743
Engineering hypervirulence in a mycoherbicidal fungus for efficient weed controlZiva Amsellem, Barry A. Cohen
& Jonathan Gressel | | | | Figure 1. The presence of NEP1 and its product in transformants.
(A) PCR determination of the presence of the NEP1 gene in various strains of Colletotrichum coccodes. The organism was co-transformed with a gene for hygromycin resistance. A representative example of three of the putative co-transformants after cultivation for four generations on hygromycin-containing medium is presented. (B) The presence of Nep1 protein in cotyledons infected by strain T-20 NEP1 Colletotrichum coccodes (western blot analysis) at two time points after inoculation.
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