Nature Biotechnology
20, 1030 - 1034 (2002)
Published online: 9 September 2002; | doi:10.1038/nbt737
Efficient gene targeting by homologous recombination in riceRie Terada1, Hiroko Urawa1, Yoshishige Inagaki1, 2, Kazuo Tsugane1
& Shigeru Iida11
National Institute for Basic Biology, Okazaki, 444-8585, Japan. 2
Current address: Faculty of Agriculture, Okayama University, Okayama, 700-8530, Japan.
Correspondence should be addressed to Shigeru Iida shigiida@nibb.ac.jpModification of genes through homologous recombination, termed gene targeting, is the most direct method to characterize gene function. In higher plants, however, the method is far from a common practice. Here we describe an efficient and reproducible procedure with a strong positive/negative selection for gene targeting in rice, which feeds more than half of the world's population and is an important model plant. About 1% of selected calli and their regenerated fertile plants were heterozygous at the targeted locus, and only one copy of the selective marker used was found at the targeted site in their genomes. The procedure's applicability to other genes will make it feasible to obtain various gene-targeted lines of rice.
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