Bio/Technology
2, 979 - 984 (1984)
doi:10.1038/nbt1184-979
Dehydration-Rehydration Vesicles: A Simple Method for High Yield Drug Entrapment in LiposomesChristopher Kirby1
& Gregory Gregoriadis1, *
1Academic Department of Medicine, Royal Free Hospital School of Medicine, University of London, Pond Street, London NW3 2QG, England.
*To whom correspondence should be addressed. A novel method of liposome preparation has been developed which is simple to use, employs mild conditions and is capable of efficient entrapment of a wide range of materials. Conditions have been established to allow optimum levels of entrapment; typically 40−50% for the protocols used here, though this can be increased by using additional lipid. The procedure is based on induction of fusion of preformed vesicles by means of dehydration and controlled rehydration. Preliminary evidence suggests that the liposomes are primarily oligo and multilamellar. Scale-up of the procedure for industrial use is expected to be straightforward.
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