Nature Biotechnology
19, 833 - 837 (2001)
doi:10.1038/nbt0901-833
Direct and sensitive detection of a human virus by rupture event scanningMatthew A. Cooper1, Fedor N. Dultsev1, Tony Minson2, Victor P. Ostanin1, Chris Abell1
& David Klenerman11
Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge CB2 1EW, United Kingdom. 2
Department of Pathology, University of Cambridge, Tennis Court Rd., CB2 1QP, United Kingdom.
Correspondence should be addressed to Matthew A. Cooper mc221@cam.ac.uk or David Klenerman dk10012@cam.ac.ukWe have developed a sensitive, economical method that directly detects viruses by making use of the interaction between type 1 herpes simplex virus (HSV1) and specific antibodies covalently attached to the oscillating surface of a quartz crystal microbalance (QCM). The virions were detached from the surface by monotonously increasing the amplitude of oscillation of the QCM, while using the QCM to sensitively detect the acoustic noise produced when the interactions were broken. We term this process rupture event scanning (REVS). The method is quantitative over at least six orders of magnitude, and its sensitivity approaches detection of a single virus particle.
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