Nature Biotechnology
19, 248 - 252 (2001)
doi:10.1038/85696
Rapid discrimination among individual DNA hairpin molecules at single-nucleotide resolution using an ion channelWenonah Vercoutere1, Stephen Winters-Hilt2, Hugh Olsen1, David Deamer1, David Haussler2
& Mark Akeson1, 21
Department of Chemistry and Biochemistry, University of California, Santa Cruz, CA 95064. 2
Center for Biomolecular Science and Engineering, University of California, Santa Cruz, CA 95064.
Correspondence should be addressed to Mark Akeson makeson@chemistry.ucsc.eduRNA and DNA strands produce ionic current signatures when driven through an  -hemolysin channel by an applied voltage. Here we combine this nanopore detector with a support vector machine (SVM) to analyze DNA hairpin molecules on the millisecond time scale. Measurable properties include duplex stem length, base pair mismatches, and loop length. This nanopore instrument can discriminate between individual DNA hairpins that differ by one base pair or by one nucleotide.
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