 | Figure 3
Nature Biotechnology
19, 71 - 74 (2001)
doi:10.1038/83559
Overexpression of the Bt cry2Aa2 operon in chloroplasts leads to formation of insecticidal crystalsBrandy De Cosa, William Moar, Seung-Bum Lee, Michael Miller
& Henry Daniell
polycistronsplastid transformationGM cropsBt resistance | | | | Figure 3. 10% SDS−PAGE gel stained with R-250 Coomassie blue.
Loaded protein concentrations are provided in parentheses. Lane 1, prestained protein standard; lane 2, partially purified Cry2Aa2 protein from E. coli (5  g); lane 3, single gene-derived Cry2Aa2 pellet extract solubilized in 50 mM NaOH (22.4 g); lane 4, single gene-derived Cry2Aa2 supernatant (66.5 g); lane 5, operon-derived Cry2Aa2 pellet extract solubilized in 50 mM NaOH (22.9 g); lane 6, operon-derived Cry2Aa2 supernatant (58.6 g); lane 7, untransformed tobacco pellet extract solubilized in 50 mM NaOH (29.8 g); lane 8, untransformed tobacco supernatant (30.4 g). Colored compounds observed in the supernatant of transgenic plants interfered with the DC Bio-Rad protein assays.
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