 | Figure 1
Nature Biotechnology
19, 71 - 74 (2001)
doi:10.1038/83559
Overexpression of the Bt cry2Aa2 operon in chloroplasts leads to formation of insecticidal crystalsBrandy De Cosa, William Moar, Seung-Bum Lee, Michael Miller
& Henry Daniell
polycistronsplastid transformationGM cropsBt resistance | | | | Figure 1. Chloroplast expression vector and PCR analysis.
(A) pLD-BD Cry2Aa2 operon (9.8 kb) with PCR primer binding sites and expected fragment sizes. PCR analysis of untransformed and putative chloroplast transformants using two primer sets: (B) 1P1M and (C) 3P3M. Lane 1, 1 kb ladder; lane 2, untransformed; lanes 3−7, pLD-BD Cry2Aa2 operon putative transformants; lane 8, pLD-BD Cry2Aa2 operon plasmid DNA.
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