 | Figure 1
Nature Biotechnology
19, 66 - 70 (2001)
doi:10.1038/83540
Lysostaphin expression in mammary glands confers protection against staphylococcal infection in transgenic miceDavid E. Kerr, Karen Plaut, A. John Bramley, Christine M. Williamson, Alistair J. Lax, Karen Moore, Kevin D. Wells
& Robert J. Wall
mastitisanimal biotechnologyantibacterial proteinStaphylococcus aureus | | | | Figure 1. Western blot analysis of lysostaphin production by transfected COS-7 cells.
(A) Construct map of cytosolically directed, native lysostaphin (Cyto-Lys). (B) Construct map of secreted, native lysostaphin (Sec-Lys). (C) Construct map of secreted, Gln125,232-lysostaphin (Sec-Gln125,232 -Lys). Proteins in cell extracts (D) or medium (E) were probed with anti-lysostaphin antiserum. Lane 1, bacterial lysostaphin (100 ng/ml). Lanes 2−5: Cells were transfected with plasmids containing the CMV promoter driving expression of hGH (lane 2), Cyto-Lys (lane 3), Sec-Lys (lane 4), or Sec-Gln125,232-Lys (lane 5).
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