Induction of human cytotoxic T lymphocytes by artificial antigen-presenting
cells
Jean-Baptiste Latouche
& Michel Sadelain
Department of Human Genetics Immunology Program, Memorial
Sloan-Kettering Cancer Center, New York, NY
10021.
Correspondence should be addressed to Michel Sadelain m-sadelain@ski.mskcc.orgadoptive cell therapyCD8+ T cellcostimulationdendritic cellsimmunotherapyretrovirus-mediated gene transfer
The adoptive transfer of antigen-specific cytotoxic T lymphocytes (CTLs)
is a promising therapeutic approach for a number of diseases. To overcome
the difficulty in generating specific CTLs, we established stable artificial
antigen-presenting cells (AAPCs) that can be used to stimulate T cells of
any patient of a given human leukocyte antigen (HLA) type. Mouse fibroblasts
were retrovirally transduced with a single HLA−peptide complex along
with the human accessory molecules B7.1, ICAM-1, and LFA-3. These AAPCs consistently
elicit strong stimulation and expansion of HLA-restricted CTLs. Owing to the
high efficiency of retrovirus-mediated gene transfer, stable AAPCs can be
readily engineered for any HLA molecule and any specific peptide.
