Nature Biotechnology
18, 1257 - 1261 (2000)
doi:10.1038/82360
A network of protein−protein interactions in yeastBenno Schwikowski1, 2, Peter Uetz3
& Stanley Fields3, 41
The Institute for Systems Biology, 4225
Roosevelt Way NE, Suite 200, Seattle, WA
98105. 2
Department of Computer Science and Engineering, University
of Washington, Box 352350, Seattle,
WA 98195. 3
Department of Genetics and Medicine, University of
Washington, Box 357360, Seattle, WA
98195. 4
Howard Hughes Medical Institute, University of Washington, Box 357360, Seattle, WA
98195.
Correspondence should be addressed to Benno Schwikowski benno@systemsbiology.org or Peter Uetz
uetz@u.washington.edu or Stanley Fields fields@u.washington.edu
Protein interactionsSaccharomyces cerevisiaefunctional genomicsproteomicsbioinformaticsA global analysis of 2,709 published interactions between proteins of the
yeast Saccharomyces cerevisiae has been performed, enabling the establishment
of a single large network of 2,358 interactions among 1,548 proteins. Proteins
of known function and cellular location tend to cluster together, with 63%
of the interactions occurring between proteins with a common functional assignment
and 76% occurring between proteins found in the same subcellular compartment.
Possible functions can be assigned to a protein based on the known functions
of its interacting partners. This approach correctly predicts a functional
category for 72% of the 1,393 characterized proteins with at least one partner
of known function, and has been applied to predict functions for 364 previously
uncharacterized proteins.
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