Applications of display technology in protein analysis
Min Li
Department of Physiology and Department of Neuroscience,
The Johns Hopkins University School of Medicine, 725 North Wolfe
Street, Baltimore, MD 21205 (
minli@jhmi.edu).
Display technology refers to a collection of methods for creating libraries
of modularly coded biomolecules that can be screened for desired properties.
It has become a routine tool for enriching molecular diversity and producing
novel types of proteins. The combination of an ever-increasing variety of
libraries of modularly coded protein complexxes with the development of innovative
approaches to select a wide array of desired properties has facilitated large-scale
analyses of protein−protein/protein−substrate interactions, rapid
isolation of antibodies (or antibody mimetics) without immunization, and function-based
protein analysis. Several practical and theoretical challenges remain to be
addressed before display technology can be readily applied to proteomic studies.
