Nature Biotechnology
18, 1177 - 1180 (2000)
doi:10.1038/81169
Immunoprophylaxis in fish by injection of mouse antibody genesNiels Lorenzen1, Pauline M. Cupit2, Katja Einer-Jensen1, Ellen Lorenzen1, Peter Ahrens3, Christopher J. Secombes4
& Charles Cunningham21
Danish Veterinary Laboratory, Hangøvej
2, DK-8200 Aarhus N, Denmark. 2
Sars International Centre for Marine Molecular Biology,
High Technology Centre, 5008 Bergen, Norway
. 3
Danish Veterinary Laboratory, Bülowsvej
27, DK-1790 V, Copenhagen, Denmark.
4
Department of Zoology, University of Aberdeen,
Aberdeen AB24 2TZ, Scotland, UK.
Correspondence should be addressed to Niels Lorenzen nl@svs.dksingle-chain antibodyrhabdovirusgene therapyAntibodies are a crucial part of the body's specific defense against infectious
diseases and have considerable potential as therapeutic and prophylactic agents
in humans and animals. The development of recombinant single-chain antibodies
allows a genetic application strategy for prevention of infectious diseases.
To test this in a fish model, a gene construct encoding a neutralizing single-chain
antibody to the fish-pathogenic rhabdovirus VHSV (viral hemorrhagic septicemia
virus) was administered to rainbow trout by intramuscular injection of plasmid
DNA. Circulating recombinant antibodies could later be detected in the fish,
and protective immunity to the viral disease was established.
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