Nature Biotechnology
18, 1080 - 1085 (2000)
doi:10.1038/80280
Minimal activators that bind to the KIX domain of p300/CBP identified by phage display screeningJohn V. Frangioni1, Leah M. LaRiccia2, Lewis C. Cantley2
& Marc R. Montminy31
The Division of Hematology/Oncology, Beth Israel Deaconess Medical Center, Boston, MA 02215. 2
The Division of Signal Transduction, Department of Medicine, Beth Israel Deaconess Medical Center, Boston, MA 02215. 3
Salk Institute, 10010 N. Torrey Pines Road, LaJolla, CA 92037.
Correspondence should be addressed to Marc R. Montminy montminy@salk.eduCREB binding proteinp300transcriptional controlphage displaygene therapyHuman gene therapy approaches involving transcription factors often rely on artificial activation domains for transcriptional activation. These domains are often large (e.g., 80 amino acids for VP16), recruit multiple co-activation complexes at once, and offer no fine control over the level of transcription. In an attempt to understand the sequence and structural requirements of a minimal mammalian activator, we employed a molecular diversity approach with a peptide phage display library composed of random eight−amino acid peptides. Using the KIX domain of the mammalian co-activators p300 and CBP as target, we discovered a family of synthetic binding peptides. These peptides share significant homology with natural KIX domain ligands, and are shown to bind an overlapping, yet distinct, surface of p300/CREB-binding protein (CBP). When fused to a heterologous DNA binding domain, these synthetic peptides function as titratable, modular, and potent transcriptional activators in living cells through specific recruitment of p300/CBP, with the level of transcriptional activation proportional to the affinity of the synthetic peptide for the KIX domain. Taken together, our data demonstrate that a molecular diversity approach can be used to discover minimal, co-activator domain-specific synthetic activators, and that transcriptional activation can be modulated as desired at the level of co-activator recruitment.
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