 | Figure 1
Nature Biotechnology
18, 75 - 80 (2000)
doi:10.1038/71958
Exploiting recombination in single bacteria to make large phage antibody
librariesDaniele Sblattero
& Andrew Bradburyantibody engineeringphage displayrecombinationCre recombinasefilamentous phagesingle-chain Fv (scFv) | | | | Figure 1. (A) Map of the display vector pDAN5 with an scFv cloned.
Sites used for V gene cloning are in bold. BssHII,
BspEI, SalI, XhoI, KpnI, NheI. (B) ELISA signals
with scFvs derived from monoclonal antibodies. Vh and Vl
genes cloned into pDAN5 in the scFv format were tested for binding to their
own antigens (D1.3: lysozyme; Y13-259: p21ras peptide; GL30: gliadin), as
well as to the other tested antigens and a control antigen: human serum albumin
(HSA).
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