Mapping signal transduction pathways by phage display
Sergey Zozulya, Mario Lioubin, Ronald J. Hill, Clare Abram
& Mikhail L. Gishizky
Sugen, Inc., 230 East Grand Ave.,
South San Francisco, CA 04080.
Correspondence should be addressed to Sergey Zozulya sergey-zozulya@sugen.com or Mikhail L. Gishizky mikhail-gishizky@sugen.comsignal transductiontyrosine phosphorylationprotein interaction screeningphage display
Rapid identification of proteins that interact with a novel gene product
is an important element of functional genomics. Here we describe a phage display−based
technique for interaction screening of complex cDNA libraries using proteins
or synthetic peptides as baits. Starting with the epidermal growth factor
receptor (EGFR) cytoplasmic tail, we identified known protein interactions
that link EGFR to the Ras/MAP kinase signal transduction cascade and several
novel interactions. This approach can be used as a rapid and efficient tool
for elucidating protein networks and mapping intracellular signal transduction
pathways.
