Nature Biotechnology
16, 642 - 646 (1998)
doi:10.1038/nbt0798-642
Trasgenic bovine chimeric offspring produced from somatic cell-derived stem-like cellsJose B. Cibelli1, 2, Stevem L. Stice2, Paul J. Golueke2, Jeff J. Kane2, Joseph Jerry1, Cathy Blackwell2, F. Abel Ponce de León2, 3
& James M. Robl1, 2, *
1Department of Veterinary and Animal Sciences, University of Massachusetts, Amherst, MA 01003.
2Advanced Cell Technology, Inc., Worcester, MA 01605.
3Animal Sciences, College of Agricultural, Food and Environmental Sciences, University of Minnesota, St Paul, MN 55108.
*e-mail: robl@vasci.umass.edu We have developed a method, using nuclear transplantation, to produce transgenic embryonic stem (ES)-like cells from fetal bovine fibroblasts. These cells, when reintroduced into preimplantation embryos, differentiated into derivatives from the three embryonic germ layers, ectoderm, mesoderm, and endo-derm, in 5-month-old animals. Six out of seven (86%) calves born were found to be chimeric for at least one tissue. These experiments demonstrate that somatic cells can be genetically modified and then de-differentiated by nuclear transfer into ES-like cells, opening the possibility of using them in differentiation studies and human cell therapy.
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