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Modification of rhizobacterial populations by engineering bacterium utilization of a novel plant-produced resource

Nature Biotechnology volume 15, pages 363–368 (1997)Cite this article

Abstract

The ability to catabolize distinct nutrients produced by a plant may be a factor in the successful colonization of that host by a bacterium when in competition with other rhizosphere microorganisms. We tested this hypothesis by examining the influence of a novel substrate produced by a transgenic plant on root colonization by near-isogenic bacteria, differing only in their ability to use the resource. When inoculated alone, both bacteria colonized the roots of the normal and transgenic plants with equal kinetics and to indistinguishable levels. When the two bacteria were coinoculated, the catabolizer reached a population density significantly higher than that of the noncatabolizer on the roots of the resource-producing plant. No such advantage was observed on the roots of normal plants. These results support the theory that resources produced and exuded by a plant host can confer a selective advantage to microorganisms that use the substrate.

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Author notes

  1. Stephen K. Farrand: Corresponding author (e-mail: stephenf@uiuc.edu).

Authors and Affiliations

  1. Department of Biology, University of West Florida, Pensacola, FL, 32514

    Michael A. Savka

  2. Departments of Crop Sciences, and Microbiology, University of Illinois at Urbana-Champaign, Urbana, IL, 61801

    Stephen K. Farrand

Authors
  1. Michael A. Savka
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  2. Stephen K. Farrand
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Savka, M., Farrand, S. Modification of rhizobacterial populations by engineering bacterium utilization of a novel plant-produced resource. Nat Biotechnol 15, 363–368 (1997). https://doi.org/10.1038/nbt0497-363

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  • DOI: https://doi.org/10.1038/nbt0497-363

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