Nature Biotechnology
15, 164 - 166 (1997)
doi:10.1038/nbt0297-164
Electrically induced NGF production by astroglial cellsSumihiro Koyama1, Tetsuya Haruyama1, Eiry Kobatake1
& Masuo Aizawa1, *
1Department of Bioengineering, Tokyo Institute of Technology, Nagatsuta, Midori-ku, Yokohama 226, Japan.
*Corresponding author (e-mail: maizawa@bio.titech.ac.jp). A controlled culture system has been developed to induce nerve growth factor (NGF) production in astroglial cells that are cultured on an electrode surface. The electrode potential is alternatively modulated at an amplitude of 300 mV and a frequency of 10 Hz. The electric stimulation triggers NGF production and secretion. The mechanism of the electrically induced NGF production is discussed in association with protein kinase C (PKC) activation.
REFERENCES
- Carman-Krzan, M., Vigé, X. and Wise, B.C. 1991. Regulation by interleukin-1 of nerve growth factor secretion and nerve growth factor mRNA expression in rat primary astroglial cultures. J. Neurochem. 56: 636−643. | PubMed | ChemPort |
- Lu, B., Yokoyama, M., Dreyfus, C.F. and Black, I.B. 1991. NGF gene expression in actively growing brain glia. J. Neurosci. 11: 318−326. | PubMed | ISI | ChemPort |
- Yoshida, K. and Gage, F.H. 1991. Fibroblast growth factors stimulate nerve growth factor synthesis and secretion by astrocytes. Brain Res. 538: 118−126. | Article | PubMed | ISI | ChemPort |
- Lindholm, D., Hengerer, B., Zafra, F. and Thoenen, H. 1990. Transforming growth factor-61 stimulates expression of nerve growth factor in the rat CMS. Neuroreport 1: 9−12. | PubMed | ChemPort |
- Kojima, J., Shinohara, H., Ikariyama, Y., Aizawa, M., Nagaike, K. and Morioka, S. 1991. Electrically controlled proliferation of human carcinoma cells cultured on the surface of an electrode. J. Biotechnol. 18: 129−140. | Article | PubMed | ISI | ChemPort |
- Kojima, J., Shinohara, H., Ikariyama, Y., Aizawa, M., Nagaike, K. and Morioka, S. 1992. Electrically promoted protein production by mammalian cells cultured on the electrode surface. Biotech. Bioeng. 39: 27−32. | ISI | ChemPort |
- Pshenichkin, S.P. and Wise, B.C. 1995. Okadaic acid increases nerve growth factor secretion, mRNA stability, and gene transcription in primary cultures of cortical astrocytes. J. Biol. Chem. 270: 5994−5999. | Article | PubMed | ISI | ChemPort |
- Tanaka, C. and Nishizuka, Y. 1994. The protein kinase C family for neuronal signaling. Annu. Rev. Neurosci. 17: 551−567. | Article | PubMed | ISI | ChemPort |
- Furukawa, Y., Furukawa, S., Omae, F., Awatsuji, H. and Hayashi, K. 1993. Alkylcatechols regulate NGF gene expression in astroglial cells via both protein kinase C- and cAMP-independent mechanisms. J. Neurosci. Res. 35: 522−529. | PubMed | ISI | ChemPort |
- Jacquier-Sarlin, M.R., Jornot, L. and Polla, B.S. 1995. Differential expression and regulation of hsp70 and hsp90 by phorbol esters and heat shock. J. Biol. Chem. 270: 14094−14099. | Article | PubMed | ChemPort |
- Hallahan, D.E., Gius, D., Kuchibhotla, J., Sukhatme, V., Kufe, D.W. and Weichselbaum, R.R. 1993. Radiation signaling mediated by Jun activation following dissociation from a cell type-specific represser. J. Biol. Chem. 268: 4903−4907. | PubMed | ISI | ChemPort |
- Yamazaki, T., Komuro, I., Kudoh, S., Zou, Y., Shiojima, I., Mizuno, T., Takano, H., Heroi, Y., Ueki, K., Tobe, K., Kadowaki, T., Nagai, R., and Yazaki, Y. 1995. Mechanical stress activates protein kinase cascade of phosphorylation in neonatal rat cardiac myocytes. J. Clin. Invest. 96: 438−446. | PubMed | ISI | ChemPort |
- Gilchrest, B.A., Park, H.-Y., Eller, M.S. and Yaar, M. 1996. Mechanisms of ultraviolet light-induced pigmentation. Photochem. Photobiol. 63: 1−10. | PubMed | ISI | ChemPort |
- Bowman, C.L., Ding, J.-R., Sachs, F. and Sokabe, M. 1992. Mechanotransducing ion channels in astrocytes. Brain Res. 584: 272−286. | Article | PubMed | ISI | ChemPort |
- Furukawa, S., Furukawa, Y., Satoyoshi, E. and Hayashi, K. 1986. Synthesis and secretion of nerve growth factor by mouse astroglial cells in culture. Biochem. Biophys. Res. Commun. 136: 57−63. | PubMed | ISI | ChemPort |
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