Nature Biotechnology
15, 1290 - 1294 (1997)
doi:10.1038/nbt1197-1290
Transgenic potato expressing a double-stranded RNA-specific ribonuclease is resistant to potato spindle tuber viroidTeruo Sano*, Akihiro Nagayama1, Toshiya Ogawa2, Isao Ishida2
& Yoshimi Okada3
1Laboratory of Phytopathology, Faculty of Agriculture, Hirosaki University, Hirosaki 036, Japan.
2Central Laboratories for Key Technology, KIRIN Brewery Co. Ltd., 1-13-5 Fukuura, Kanazawa-ku, Yokohama 236, Japan
3Department of Biosciences, School of Science and Engineering, Teikyo University, Toyosato-dai 1-1, Utsunomiya 320, Japan.
*e-mail: sano@owani8.cc.hirosaki-u.ac.jp. We have produced transgenic potato lines expressing the yeast-derived double-stranded RNA-specific ribonuclease pac1. Five lines of pac1 potato (Solanum tuberosum L, cultivar Russet Burbank) challenged with potato spindle tuber viroid (PSTVd) suppressed PSTVd infection and accumulation. All of the progeny potato tubers produced by resistant plants were also free of PSTVd. Because the pac1 gene product digested PSTVd in vitro, double-stranded regions in PSTVd molecule and/or replicative intermediates may be targeted by pac1 gene product in the transgenic potato plant.
REFERENCES
- Diener, T.O. 1987. The viroids. Plenum Publishing Corporation, New York.
- Powell, P.A., Nelson, R.S., De, B., Hoffman, N., Roger, S.G., Fraley, R.T., and Beachy, R.N. 1986. Delay of disease development in transgenic plants that express the tobacco mosaic virus coat protein gene. Science 232: 738−743. | PubMed |
- Golemboski, D.B., Lomonossoff, G.P., and Zaitlin, M. 1990. Plants transformed with a tobacco mosaic virus nonstructural sequence are resistant to the virus. Proc. Natl. Acad. Sci. USA 87: 6311−6315. | PubMed | ChemPort |
- Lapidot, M., Gafney, R., Ding, B., Wolf, S., Lucas, W.J., and Beachy, R.N. 1993. A dysfunctional movement protein of tobacco mosaic virus that partially modifies the plasmodesmata and limits virus spread in transgenic plants. Plant J. 4: 959−970. | Article | ISI | ChemPort |
- Maiti, I.B., Murphy, J.F., Shaw, J.G., and Hunt, A.G. 1993. Plants that express a potyvirus proteinase gene are resistant to virus infection. Proc. Natl. Acad. Sci. USA 90: 6110−6114. | PubMed | ChemPort |
- Powell, P.A., Stark, D.M., Sanders, P.R., and Beachy, R.N. 1989. Protection against tobacco mosaic virus in transgenic plants that express tobacco mosaic virus antisense RNA. Proc. Natl. Acad. Sci. USA 86: 6949−6952. | PubMed | ChemPort |
- Beachy, R.N., Loesch-Fries, S., and Tumer, N.E. 1990. Coat protein-mediated resistance against virus infection. Annu. Rev. Phytopathol. 28: 451−474. | Article | ISI | ChemPort |
- Lindbo, J.A. and Dougherty, W.G. 1992. Untranslatable transcripts of the tobacco etch virus coat protein gene sequence can interfere with tobacco etch virus replication in transgenic plants and protoplasts. Virology 189: 725−733. | Article | PubMed | ISI | ChemPort |
- Lomonossoff, G.P. 1995. Pathogen-derived resistance of plant viruses. Annu. Rev. Phytopathol. 33: 323−343. | Article | ISI | ChemPort |
- Atkins, D., Young, M., Uzzell, S., Kelly, L., Fillatti, J., and Gerlach, W.L. 1995. The expression of antisense and ribozyme genes targeting citrus exocortis viroid in transgenic plants. J. Gen. Virol. 76: 1781−1790. | PubMed | ISI | ChemPort |
- Matousek, J., Schroder, A.R.W., Trnena, L., Reimers, M., Baumstark, T., Dedic, P., Vlasak, J., et al. 1994. Inhibition of viroid infection by antisense RNA expression in transgenic plants. Biol. Che. Hoppe Seyler 375: 765−777. | ISI | ChemPort |
- lino, Y., Sugimoto, A., and Yamamoto, M.S. 1991. pombe pac1+, whose over expression inhibits sexual development, encodes a ribonuclease III-like RNase. EMBO J. 10: 221−226. | PubMed | ISI | ChemPort |
- Watanabe, Y., Ogawa, T., Takahashi, H., Ishida, I., Takeuchi, Y., Yamamoto, M., and Okada, Y. 1995. Resistance against multiple plant viruses in plants mediated by a double stranded-RNA specific ribonuclease. FEBS Lett. 372: 165−168. | Article | PubMed | ISI | ChemPort |
- Branch, A.D. and Dickson, E. 1984. A replication cycle for viroids and other small infectious RNA's. Science 223: 450−455. | PubMed | ISI | ChemPort |
- Ishikawa, M., Meshi, T., Ohno, T., Okada, Y., Sano, T., Ueda, I., and Shikata, E. 1984. A revised replication cycle for viroids: The role of longer than unit length RNA in viroid replication. Mol. Gen. Genet. 196: 421−428. | PubMed | ISI | ChemPort |
- Gross, H.J., Domdey, H., Lossow, C., Jank, P., Raba, M., Alberty, H., and Sänger, H.L. 1978. Nucleotide sequence and secondary structure of potato spindle tuber viroid. Nature 273: 203−208. | PubMed | ISI | ChemPort |
- Riesner, D. 1987. Physical-chemical properties; structure formation, pp. 63−116 in The viroids. Diener, T.O. (ed.) Plenum Publishing Corporation, New York.
- Qu, F., Heinrich, C., Loss, P., Steger, G., Tien, P., and Riesner, D. 1993. Multiple pathways of reversion in viroids for conservation of structural elements. EMBO J. 12: 2129−2139. | PubMed | ISI | ChemPort |
- Sänger, H.L. 1987. Viroid function; viroid replication, pp. 117−166 in The viroids. Diener, T.O. (ed.) Plenum Publishing Corporation, New York.
- Owens, R.A. and Diener, T.O. 1982. RNA intermediates in potato spindle tuber viroid replication. Proc. Natl. Acad. Sci. USA 79: 113−117. | ChemPort |
- Riesner, D. 1990. Structure of viroids and their replication intermediates. Are thermodynamic domains also functional domains? Seminars in Virology 1: 83−99.
- Symons, R.H. 1990. Self-cleavage of RNA in the replication of viroids and viru-soids. Seminars in Virology 1: 117−126.
- Sano, T., and Singh, R.P. 1995. Avocado sunblotch viroid group, pp. 363−371 in Pathogenesis and host specificity in plant diseases, vol. III: viruses and viroids. Singh, U.S. (ed.) Pergamon/Elsevier Science Ltd., Oxford, UK.
- Bonfiglioli, R.G., MacFadden, G.I., and Symons, R.H. 1994. In situ hybridization localizes avocado sunblotch viroid on chloroplast thylakoid membranes and coconut cadang cadang viroid in the nucleus. Plant J. 6: 99−103. | Article | ISI |
- Estrada, P., Tovar, P., and Didds, J.H. 1986. Induction of in vitro tubers in a broad range of potato genotypes. Plant Cell Tissue Organ Cult. 7: 3−10. | ISI |
- Ishida, B.K., Snyder, G.W., and Belknap, W.R. 1989. The use of in vitro-grown microtuber discs in Agrobacterium-mediated transformation of Russet Burbank and Lemhi Russet potatoes. Plant Cell Rep. 8: 325−328. | ISI | ChemPort |
- Murashige, T., and Skoog, F. 1962. A revised medium for rapid growth and bioas-says with tobacco tissue cultures. Physiol. Plant 15: 473−497. | ISI | ChemPort |
- Li, S., Onodera, S., Sano, T., Yoshida, K., Wang, G., and Shikata, E. 1995. Gene diagnosis of viroids: comparisons of return-PAGE and hybridization using DIG-labeled DNA and RNA probes for practical diagnosis of hop stunt, citrus exocortis and apple scar skin viroids in their natural host plants. Ann. Phytopathol. Soc. Jpn. 61: 381−390. | ChemPort |
- Singh, R.P. and Boucher, A. 1987. Electrophoretic separation of a severe from mild strains of potato spindle tuber viroid. Phytopathology 77: 1588−1591. | ISI |
- Sano, T. and Ishiguro, A. 1996. A simple and sensitive non-radioactive microplate hybridization for the detection and quantification of picograms of viroid and viral RNA. Arch. Phytopath. Pflanz. 30: 303−312. | ChemPort |
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