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Research Article
Nature Biotechnology  14, 1021 - 1025 (1996)
doi:10.1038/nbt0896-1021

An enzyme-linked oligonucleotide assay

Daniel W. Drolet1, *, Lotus Moon-McDermott1 & Timothy S. Romig1

  1NeXstar Pharmaceuticals, 2860 Wilderness Place, Boulder, CO 80301, USA

  *e-mail: ddrolet@nexstar.com

The recent development of in vitro methods to select high-affinity ligands by combinatorial chemistry methodologies promises unique and theoretically unlimited supplies of novel therapeutic and diagnostic reagents. One such combinatorial chemistry process, systematic evolution of ligands by exponential enrichment (SELEX), allows rapid identification, from large random sequence pools, of the few oligonucleotide sequences that bind to a desired target molecule with high affinity and specificity. We describe an enzyme-linked sandwich assay that uses a SELEX-derived oligonucleotide. This assay demonstrates that these oligonucleotides can be effective and useful analytical reagents.

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EISSN: 1546-1696
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