Nature Biotechnology
14, 760 - 764 (1996)
doi:10.1038/nbt0696-760
pH-sensitive, cationic liposomes: A new synthetic virus-like vectorVladimir Budker1, Vladimir Gurevich1, James E. Hagstrom1, Fedor Bortzov1
& Jon A. Wolff1, *
1Departments of Pediatrics and Medical Genetics, Waisman Center, University of Wisconsin, Madison, WI 53705.
*e-mail:wolffj@macc.wisc.edu We describe the use of cationic, pH-sensitive liposomes to mediate the efficient transfer of DNA into a variety of cells in culture. Cationic lipids, containing an amine with a pK within the physiologic range of 4.5 to 8, were synthesized and incorporated with dioleoylphosphatidylethanolamine into liposomes. Acid conditions promoted DNA-binding, DNA-incorporation, and DNA-induced fusion by these cationic, pH-sensitive liposomes. Transfection efficiency in cultured cells was dependent on endosomal acidification in a manner akin to acidic-induced endosomal release of viruses. These liposomes constitute a promising new class of reagents for gene therapy. REFERENCES
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