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Research Article
Nature Biotechnology  14, 1587 - 1591 (1996)
doi:10.1038/nbt1196-1587

Inactivation of MET10 in brewer's yeast specifically increases SO2 formation during beer production

Jørgen Hansen1, 2, * & Morten C. Kielland-Brandt2

  1Carlsberg Research Laboratory, Gamle Carlsberg Vej 10, DK-2500 Copenhagen Valby, Denmark.

  2Carlsberg Laboratory, Department of Yeast Genetics, Gamle Carlsberg Vej 10, DK-2500 Copenhagen Valby, Denmark.

  *e-mail: carlgaer@biobase.dk

Sulfite is widely used as an antioxidant in food production. In beer brewing, sulfite has the additional role of stabilizing the flavor by forming adducts with aldehydes. Inadequate amounts of sulfite are sometimes produced by brewer's yeasts, so means of controlling the sulfite production are desired. In Saccharomyces yeasts, MET10 encodes a subunit of sulfite reductase. Partial or full elimination of MET10 gene activity in a brewer's yeast resulted in increased sulfite accumulation. Beer produced with such yeasts was quite satisfactory and showed increased flavor stability.

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ISSN: 1087-0156
EISSN: 1546-1696
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