Research abstract


Nature Biotechnology 13, 686 - 691 (1995)
doi:10.1038/nbt0795-686

Genetic Engineering of Rice for Resistance to Sheath Blight

Willie Lin1, C.S. Anuratha1, Karabi Datta2, ,3, Ingo Potrykus3, S. Muthukrishnan1, ,* & Swapan K. Datta2, ,3


A 1.1 kb rice genomic DNA fragment, containing a chitinase gene under the control of the CaMV 35S promoter, was cloned into the rice transformation vector pGL2. After transformation of Indica rice protoplasts in the presence of polyethyleneglycol, plants were regenerated. The presence of the chimeric chitinase gene in T0 and T1 transgenic rice plants was detected by Southern blot analysis. Western blot analysis of transgenic plants and their progeny revealed the presence of two proteins with apparent molecular weights of 30 and 35 kDa that reacted with the chitinase antibody. Progeny from the chitinase-positive plants were tested for their resistance to the sheath blight pathogen, Rhizoctonia solani. The degree of resistance displayed by the transgenic plants to this pathogen correlated with the level of chitinase expression.

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  1. 1Department of Biochemistry, Kansas State University, Manhattan, Kansas 66506.
  2. 2International Rice Research Institute, P.O. Box 933, 1099 Manila, The Philippines.
  3. 3ETH-Zurich, Institute of Plant Sciences, Zurich CH-8092, Switzerland.
  4. *email: smk@ksuvm.ksu.edu and sdatta@irri.cgnet.com