Bio/Technology
10, 1030 - 1035 (1992)
doi:10.1038/nbt0992-1030
Oligonucleotide and Amplification Fingerprinting of Wild Species and Cultivars of Banana (Musa spp.)Dieter Kaemmer1, Rownak Afza2, Kurt Weising1, Günter Kahl1
& Frantisek J. Novak1, *
1Plant Molecular Biology Group, University of Frankfurt/Main, Department of Biology, W-6000 Frankfurt/Main, Germany.
2Plant Breeding Unit, Joint FAO/IAEA Programme, International Atomic Energy Agency, Seibersdorf Laboratories, P.O. Box 100, A-1400 Vienna, Austria.
*Corresponding author. DNA oligonucleotide and amplification fingerprinting have been successfully used to detect genetic polymorphisms in 15 representative species and cultivars of the genus Musa, comprising AA, AAA, AAAA, AAB, ABB, and BB genotypes. In−gel−hybridization of Hinf I−digested genomic banana DNA to the 32P−labeled synthetic oligonucleotides (GATA)4, (GTG)5, and (CA)8 revealed considerable polymorphisms between Musa species and cultivars. The fingerprint patterns proved to be somatically stable and did not show differences between individual plants of "Grand Nain" (AAA genotype). Dendrograms based on oligonucleotide fingerprint band sharing data proved to be consistent with most of the known features of the history of banana and plantain cultivation and evolution, respectively. DNA samples from the same banana species and cultivars were also amplified by PCR using single or pairwise combinations of short oligonucleotide primers. Amplification products were separated on agarose or polyacrylamide gels and visualized by ethidium bromide or silver staining, respectively. Polymorphic patterns were obtained with some but not all primers. By using the CCCTCTGCGG primer in simplex and/or duplex PCR, the induced mutant 'GN60A' was clearly recognized from its original variety 'Grand Nain'. Both fingerprint techniques allowed the detection of bands characteristic for the A and B genome. This DNA fingerprinting technology has potential application in several areas of Musa improvement.
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