Research Papers abstract

Nature Biotechnology 10, 176 - 181 (1992)

Growth Enhancement in Transgenic Atlantic Salmon by the Use of an "All Fish" Chimeric Growth Hormone Gene Construct

Shao Jun Du1, Zhiyuan Gong1, Garth L. Fletcher2, Margaret A. Shears2, Madonna J. King2, David R. Idler2 & Choy L. Hew1, ,*

We have developed an "all fish" growth hormone (GH) chimeric gene construct by using an antifreeze protein gene (AFP) promoter from ocean pout linked to a chinook salmon GH cDNA clone. After microinjection into fertilized, nonactivated Atlantic salmon eggs via the micropyle, transgenic Atlantic salmon were generated. The presence of the transgene was detected by polymerase chain reaction (PCR) using specific oligonucleotide primers. A number of these transgenic fish showed dramatic increases in their growth rate. At one year old, the average increase of the transgenic fish was 2 to 6 fold and the largest transgenic fish was 13 times that of the average non-transgenic control.

  1. 1Research Institute, The Hospital for Sick Children and Departments of Clinical Biochemistry and Biochemistry, University of Toronto, Toronto, Canada M5G 1L5.
  2. 2Ocean Sciences Centre, Memorial University of Newfoundland, St. John's, Newfoundland, Canada A1C 557.
  3. *Corresponding author.