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High Speed Automated DNA Sequencing in Ultrathin Slab Gels

Bio/Technology volume 10, pages 78–81 (1992)Cite this article

Abstract

We have developed a high speed instrument for automated DNA sequence analysis. The apparatus employs laser excitation and a cooled CCD detector for the parallel detection of up to 18 sets of four fluorescently labeled DNA sequencing reactions during their electrophoretic separation in ultrathin (50–100 μm) denaturing polyacrylamide gels. Four hundred and fifty bases of sequence information is obtained from 100 ng of M13 template DNA in less than one hour, corresponding to an overall instrument throughput of over 8000 bases/hr.

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Authors and Affiliations

  1. Department of Chemistry, University of Wisconsin-Madison, Madison, WI, 53706

    Anthony J. Kostichka, Michael L. Marchbanks, Robert L. Brumley Jr., Howard Drossman & Lloyd M. Smith

Authors
  1. Anthony J. Kostichka
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  2. Michael L. Marchbanks
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  3. Robert L. Brumley Jr.
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  4. Howard Drossman
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  5. Lloyd M. Smith
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Kostichka, A., Marchbanks, M., Brumley, R. et al. High Speed Automated DNA Sequencing in Ultrathin Slab Gels. Nat Biotechnol 10, 78–81 (1992). https://doi.org/10.1038/nbt0192-78

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