Articles in 2014

  • Article |

    An unbiased approach for the genome-wide detection of off-target cleavage by CRISPR-Cas9 RNA–guided nucleases reveals wide variability in the off-target activity of different guide RNAs.

    • Shengdar Q Tsai
    • , Zongli Zheng
    • , Nhu T Nguyen
    • , Matthew Liebers
    • , Ved V Topkar
    • , Vishal Thapar
    • , Nicolas Wyvekens
    • , Cyd Khayter
    • , A John Iafrate
    • , Long P Le
    • , Martin J Aryee
    •  & J Keith Joung
  • Analysis |

    Combining four years of field data with computer modeling reveals that development of resistance to Bacillus thuringiensis insecticidal proteins (Bt) in cotton bollworm can be delayed by refuges of non-Bt host plants other than cotton, but that these so-called ‘natural refuges’ are not as effective as non-Bt cotton refuges.

    • Lin Jin
    • , Haonan Zhang
    • , Yanhui Lu
    • , Yihua Yang
    • , Kongming Wu
    • , Bruce E Tabashnik
    •  & Yidong Wu
  • Editorial |

    Mandatory labeling of GM food in the United States will not only make all food more costly but also bamboozle consumers.

  • Bioentrepreneur |

    US university technology transfer offices are adopting new models in search of increased return on research investment.

    • Brady Huggett
  • News Feature |

    As US legislatures and voters continue to wrestle with GM labeling, what is at stake? Jim Kling investigates.

    • Jim Kling
  • News & Views |

    Delivery of siRNA into cells is achieved by neutralizing the negative charge of the phosphate backbone in a reversible manner.

    • Anastasia Khvorova
    • , Maire F Osborn
    •  & Matthew R Hassler
  • News & Views |

    A new biological device known as a 'load driver' improves the performance of synthetic circuits by insulating genetic parts from each other.

    • Eric Klavins
  • Careers and Recruitment |

  • Patents |

    Recent developments in the prosecution of the CRISPR-Cas9 patent in Europe highlight the differences between the intellectual property regimes of Europe and the United States.

    • András Kupecz
  • Letter |

    Liu et al. provide new resources for improving rice by cloning a gene cluster that enhances resistance to two species of planthoppers, which cause billions of dollars of crop loss.

    • Yuqiang Liu
    • , Han Wu
    • , Hong Chen
    • , Yanling Liu
    • , Jun He
    • , Haiyan Kang
    • , Zhiguang Sun
    • , Gen Pan
    • , Qi Wang
    • , Jinlong Hu
    • , Feng Zhou
    • , Kunneng Zhou
    • , Xiaoming Zheng
    • , Yulong Ren
    • , Liangming Chen
    • , Yihua Wang
    • , Zhigang Zhao
    • , Qibing Lin
    • , Fuqing Wu
    • , Xin Zhang
    • , Xiuping Guo
    • , Xianian Cheng
    • , Ling Jiang
    • , Chuanyin Wu
    • , Haiyang Wang
    •  & Jianmin Wan
  • Resource |

    A comprehensive analysis of RNA sequencing and single-nucleotide polymorphism (SNP) array data provides new insights into the biology of 675 human cancer cell lines

    • Christiaan Klijn
    • , Steffen Durinck
    • , Eric W Stawiski
    • , Peter M Haverty
    • , Zhaoshi Jiang
    • , Hanbin Liu
    • , Jeremiah Degenhardt
    • , Oleg Mayba
    • , Florian Gnad
    • , Jinfeng Liu
    • , Gregoire Pau
    • , Jens Reeder
    • , Yi Cao
    • , Kiran Mukhyala
    • , Suresh K Selvaraj
    • , Mamie Yu
    • , Gregory J Zynda
    • , Matthew J Brauer
    • , Thomas D Wu
    • , Robert C Gentleman
    • , Gerard Manning
    • , Robert L Yauch
    • , Richard Bourgon
    • , David Stokoe
    • , Zora Modrusan
    • , Richard M Neve
    • , Frederic J de Sauvage
    • , Jeffrey Settleman
    • , Somasekar Seshagiri
    •  & Zemin Zhang
  • Analysis |

    A comparison of different ways of generating induced pluripotent stem cells helps researchers choose the most appropriate method for particular applications.

    • Thorsten M Schlaeger
    • , Laurence Daheron
    • , Thomas R Brickler
    • , Samuel Entwisle
    • , Karrie Chan
    • , Amelia Cianci
    • , Alexander DeVine
    • , Andrew Ettenger
    • , Kelly Fitzgerald
    • , Michelle Godfrey
    • , Dipti Gupta
    • , Jade McPherson
    • , Prerana Malwadkar
    • , Manav Gupta
    • , Blair Bell
    • , Akiko Doi
    • , Namyoung Jung
    • , Xin Li
    • , Maureen S Lynes
    • , Emily Brookes
    • , Anne B C Cherry
    • , Didem Demirbas
    • , Alexander M Tsankov
    • , Leonard I Zon
    • , Lee L Rubin
    • , Andrew P Feinberg
    • , Alexander Meissner
    • , Chad A Cowan
    •  & George Q Daley
  • Article |

    Combinatorial replacement and rearrangement of genetic parts in a refactored nitrogen fixation gene cluster lead to improved performance.

    • Michael J Smanski
    • , Swapnil Bhatia
    • , Dehua Zhao
    • , YongJin Park
    • , Lauren B A Woodruff
    • , Georgia Giannoukos
    • , Dawn Ciulla
    • , Michele Busby
    • , Johnathan Calderon
    • , Robert Nicol
    • , D Benjamin Gordon
    • , Douglas Densmore
    •  & Christopher A Voigt
  • Letter
    | Open Access

    The genome sequence of the ferret, a model of human respiratory disease, enables research on influenza and cystic fibrosis.

    • Xinxia Peng
    • , Jessica Alföldi
    • , Kevin Gori
    • , Amie J Eisfeld
    • , Scott R Tyler
    • , Jennifer Tisoncik-Go
    • , David Brawand
    • , G Lynn Law
    • , Nives Skunca
    • , Masato Hatta
    • , David J Gasper
    • , Sara M Kelly
    • , Jean Chang
    • , Matthew J Thomas
    • , Jeremy Johnson
    • , Aaron M Berlin
    • , Marcia Lara
    • , Pamela Russell
    • , Ross Swofford
    • , Jason Turner-Maier
    • , Sarah Young
    • , Thibaut Hourlier
    • , Bronwen Aken
    • , Steve Searle
    • , Xingshen Sun
    • , Yaling Yi
    • , M Suresh
    • , Terrence M Tumpey
    • , Adam Siepel
    • , Samantha M Wisely
    • , Christophe Dessimoz
    • , Yoshihiro Kawaoka
    • , Bruce W Birren
    • , Kerstin Lindblad-Toh
    • , Federica Di Palma
    • , John F Engelhardt
    • , Robert E Palermo
    •  & Michael G Katze
  • Analysis |

    A community computational challenge generates algorithms to predict activity of drug combinations.

    • Mukesh Bansal
    • , Jichen Yang
    • , Charles Karan
    • , Michael P Menden
    • , James C Costello
    • , Hao Tang
    • , Guanghua Xiao
    • , Yajuan Li
    • , Jeffrey Allen
    • , Rui Zhong
    • , Beibei Chen
    • , Minsoo Kim
    • , Tao Wang
    • , Laura M Heiser
    • , Ronald Realubit
    • , Michela Mattioli
    • , Mariano J Alvarez
    • , Yao Shen
    • , Jean-Paul Abbuehl
    • , Jeffrey Allen
    • , Russ B Altman
    • , Shawn Balcome
    • , Mukesh Bansal
    • , Ana Bell
    • , Andreas Bender
    • , Bonnie Berger
    • , Jonathan Bernard
    • , Andrew A Bieberich
    • , Giorgos Borboudakis
    • , Andrea Califano
    • , Christina Chan
    • , Beibei Chen
    • , Ting-Huei Chen
    • , Jaejoon Choi
    • , Luis Pedro Coelho
    • , James C Costello
    • , Chad J Creighton
    • , Will Dampier
    • , V Jo Davisson
    • , Raamesh Deshpande
    • , Lixia Diao
    • , Barbara Di Camillo
    • , Murat Dundar
    • , Adam Ertel
    • , Cellworks Group
    • , Daniel Gallahan
    • , Chirayu P Goswami
    • , Assaf Gottlieb
    • , Michael N Gould
    • , Jonathan Goya
    • , Michael Grau
    • , Joe W Gray
    • , Laura M Heiser
    • , Hussein A Hejase
    • , Michael F Hoffmann
    • , Krisztian Homicsko
    • , Max Homilius
    • , Woochang Hwang
    • , Adriaan P Ijzerman
    • , Olli Kallioniemi
    • , Bilge Karacali
    • , Charles Karan
    • , Samuel Kaski
    • , Junho Kim
    • , Minsoo Kim
    • , Arjun Krishnan
    • , Junehawk Lee
    • , Young-Suk Lee
    • , Eelke B Lenselink
    • , Peter Lenz
    • , Lang Li
    • , Jun Li
    • , Yajuan Li
    • , Han Liang
    • , Michela Mattioli
    • , Michael P Menden
    • , John-Patrick Mpindi
    • , Chad L Myers
    • , Michael A Newton
    • , John P Overington
    • , Juuso Parkkinen
    • , Robert J Prill
    • , Jian Peng
    • , Richard Pestell
    • , Peng Qiu
    • , Bartek Rajwa
    • , Ronald Realubit
    • , Anguraj Sadanandam
    • , Julio Saez-Rodriguez
    • , Francesco Sambo
    • , Dinah Singer
    • , Gustavo Stolovitzky
    • , Arvind Sridhar
    • , Wei Sun
    • , Hao Tang
    • , Gianna M Toffolo
    • , Aydin Tozeren
    • , Olga G Troyanskaya
    • , Ioannis Tsamardinos
    • , Herman W T van Vlijmen
    • , Tao Wang
    • , Wen Wang
    • , Joerg K Wegner
    • , Krister Wennerberg
    • , Gerard J P van Westen
    • , Tian Xia
    • , Guanghua Xiao
    • , Yang Xie
    • , Jichen Yang
    • , Yang Yang
    • , Victoria Yao
    • , Yuan Yuan
    • , Haoyang Zeng
    • , Shihua Zhang
    • , Junfei Zhao
    • , Jian Zhou
    • , Rui Zhong
    • , Daniel Gallahan
    • , Dinah Singer
    • , Julio Saez-Rodriguez
    • , Yang Xie
    • , Gustavo Stolovitzky
    •  & Andrea Califano
  • Letter |

    Neutralizing the RNA phosphodiester backbone enables delivery of siRNA across cell membranes.

    • Bryan R Meade
    • , Khirud Gogoi
    • , Alexander S Hamil
    • , Caroline Palm-Apergi
    • , Arjen van den Berg
    • , Jonathan C Hagopian
    • , Aaron D Springer
    • , Akiko Eguchi
    • , Apollo D Kacsinta
    • , Connor F Dowdy
    • , Asaf Presente
    • , Peter Lönn
    • , Manuel Kaulich
    • , Naohisa Yoshioka
    • , Edwige Gros
    • , Xian-Shu Cui
    •  & Steven F Dowdy
  • Article |

    Efficient reprogramming of pancreatic acinar cells in vivo generates cells that mature toward a beta cell phenotype over many months.

    • Weida Li
    • , Claudia Cavelti-Weder
    • , Yingying Zhang
    • , Kendell Clement
    • , Scott Donovan
    • , Gabriel Gonzalez
    • , Jiang Zhu
    • , Marianne Stemann
    • , Ke Xu
    • , Tatsu Hashimoto
    • , Takatsugu Yamada
    • , Mio Nakanishi
    • , Yuemei Zhang
    • , Samuel Zeng
    • , David Gifford
    • , Alexander Meissner
    • , Gordon Weir
    •  & Qiao Zhou
  • Letter |

    An orthogonal protein degradation system in bacteria provides control of both protein concentration and the protein degradation rate.

    • D Ewen Cameron
    •  & James J Collins
  • News & Views |

    The molecular and cellular architecture of the organs in a whole mouse is revealed through optical clearing.

    • Burkhard Höckendorf
    • , Luke D Lavis
    •  & Philipp J Keller
  • Erratum |

    • Sheng Li
    • , Scott W Tighe
    • , Charles M Nicolet
    • , Deborah Grove
    • , Shawn Levy
    • , William Farmerie
    • , Agnes Viale
    • , Chris Wright
    • , Peter A Schweitzer
    • , Yuan Gao
    • , Dewey Kim
    • , Joe Boland
    • , Belynda Hicks
    • , Ryan Kim
    • , Sagar Chhangawala
    • , Nadereh Jafari
    • , Nalini Raghavachari
    • , Jorge Gandara
    • , Natàlia Garcia-Reyero
    • , Cynthia Hendrickson
    • , David Roberson
    • , Jeffrey Rosenfeld
    • , Todd Smith
    • , Jason G Underwood
    • , May Wang
    • , Paul Zumbo
    • , Don A Baldwin
    • , George S Grills
    •  & Christopher E Mason
  • News Feature |

    As mass spectrometry makes inroads into pathogen identification in the clinical laboratory, deep sequencing—even nanopore sequencing—is waiting in the wings. Jeffrey L. Fox investigates.

    • Jeffrey L Fox