10 January 2002

Proteomics: Functional proteomics in yeast

Two groups present the results of large-scale functional proteomics studies of the yeast Saccharomyces cerevisiae. In a collaboration between Cellzome, EMBL and CGM-CNRS, over 1,700 proteins were tagged by homologous recombination, 1,143 of them relevant to human biology. Mass spectrometry of the gene products identified 232 multiprotein complexes and possible new cellular roles for 344 proteins. Another group including workers at MDS Proteomics and at three Canadian institutions analysed over 10% of predicted yeast proteins as baits and detected 3,617 associated proteins covering 25% of the genome.

Functional organization of the yeast proteome by systematic analysis of protein complexes
ANNE-CLAUDE GAVIN, MARKUS B�SCHE, ROLAND KRAUSE, PAOLA GRANDI, MARTINA MARZIOCH, ANDREAS BAUER, J�RG SCHULTZ, JENS M. RICK, ANNE-MARIE MICHON, CRISTINA-MARIA CRUCIAT, MARITA REMOR, CHRISTIAN H�FERT, MALGORZATA SCHELDER, MIRO BRAJENOVIC, HEINZ RUFFNER, ALEJANDRO MERINO, KARIN KLEIN, MANUELA HUDAK, DAVID DICKSON, TATJANA RUDI, VOLKER GNAU, ANGELA BAUCH, SONJA BASTUCK, BETTINA HUHSE, CHRISTINA LEUTWEIN, MARIE-ANNE HEURTIER, RICHARD R. COPLEY, ANGELA EDELMANN, ERICH QUERFURTH, VLADIMIR RYBIN, GERARD DREWES, MANFRED RAIDA, TEWIS BOUWMEESTER, PEER BORK, BERTRAND SERAPHIN, BERNHARD KUSTER, GITTE NEUBAUER & GIULIO SUPERTI-FURGA
Nature 415, 141–147 (10 January 2002)
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Systematic identification of protein complexes in Saccharomyces cerevisiae by mass spectrometry
YUEN HO, ALBRECHT GRUHLER, ADRIAN HEILBUT, GARY D. BADER, LYNDA MOORE, SALLY-LIN ADAMS, ANNA MILLAR, PAUL TAYLOR, KEIRYN BENNETT, KELLY BOUTILIER, LINGYUN YANG, CHERYL WOLTING, IAN DONALDSON, SØREN SCHANDORFF, JUANITA SHEWNARANE, MAI VO, JOANNE TAGGART, MARILYN GOUDREAULT, BRENDA MUSKAT, CRIS ALFARANO, DANIELLE DEWAR, ZHEN LIN, KATERINA MICHALICKOVA, ANDREW R. WILLEMS, HOLLY SASSI, PETER A. NIELSEN, KARINA J. RASMUSSEN, JENS R. ANDERSEN, LENE E. JOHANSEN, LYKKE H. HANSEN, HANS JESPERSEN, ALEXANDRE PODTELEJNIKOV, EVA NIELSEN, JANNE CRAWFORD, VIBEKE POULSEN, BIRGITTE D. SØRENSEN, JESPER MATTHIESEN, RONALD C. HENDRICKSON, FRANK GLEESON, TONY PAWSON, MICHAEL F. MORAN, DANIEL DUROCHER, MATTHIAS MANN, CHRISTOPHER W. V. HOGUE, DANIEL FIGEYS & MIKE TYERS
Nature 415, 180–183 (10 January 2002)
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Protein complexes take the bait
ANUJ KUMAR & MICHAEL SNYDER
Many cellular functions are carried out by proteins that are bound together in complexes. In two new large-scale studies, labelled proteins are used as 'bait' to capture and identify those complexes.
Nature 415, 123–124 (10 January 2002)
| Full Text | PDF (84 K) |