Present address: Conservatoire National des Arts et Métiers, 75003 Paris, France.
- Charly Empereur-Mot
Department of Structural Biology, Weizmann Institute of Science, Rehovot 7610001, Israel
- Hector Garcia-Seisdedos,
- Charly Empereur-Mot &
- Emmanuel D. Levy
Department of Chemical Research Support, Weizmann Institute of Science, Rehovot 7610001, Israel
- Nadav Elad
H.G.S. and E.D.L. designed the experiments. C.E.M. and E.D.L. designed the computational analyses of protein structure. H.G.S. performed all experiments with guidance from N.E. for electron microscopy techniques. N.E. and H.G.S. performed the single-particle reconstruction. C.E.M. performed the bioinformatics analyses. H.G.S. and E.D.L. analysed the experimental data. C.E.M. and E.D.L. analysed the computational results. E.D.L. and H.G.S. wrote the manuscript with help from all authors.
Competing financial interests
The authors declare no competing financial interests.
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Emmanuel D. Levy
Extended Data Figure 1: Increasing surface hydrophobicity of a homo-oligomer triggers its supramolecular self-assembly.Hover over figure to zoom
Extended Data Figure 2: The proteins studied exhibit intracellular concentrations situated within a physiological range.Hover over figure to zoom
Extended Data Figure 3: Circular dichroism spectra of all wild-type and mutant pairs studied.Hover over figure to zoom
Extended Data Figure 4: Characterization of protein fibres by electron microscopy.Hover over figure to zoom
Extended Data Figure 5: Single-particle three-dimensional reconstruction of the filament formed by the dipeptidase.Hover over figure to zoom
Extended Data Figure 6: Detailed view of the helix–helix interface driving the formation of the dipeptidase fibre.Hover over figure to zoom
Extended Data Figure 7: Stickiness is tuned as a function of nDp in dihedral complexes but not in cyclic complexes.Hover over figure to zoom
Extended Data Figure 8: Self-assembly takes place at low concentrations in vitro.Hover over figure to zoom
Video 1: Cells divide while expressing the fiber-forming mutant of E. coli dipeptidase (1POK E239Y)
Extended Data Table 1: Protein structures originally selected for mutationHover over figure to zoom
Extended Data Table 2: Size and assembly characteristics of the fibres observed in vitroHover over figure to zoom