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Extended data figures and tables
Extended Data Figures
- Extended Data Figure 1: Sequence alignment of SMO orthologues. (1,355 KB)
Numbering corresponds to that of human SMO. Secondary structure assignments are displayed above the alignment and colour-coded as in Fig. 1. Black arrows and numbers (fX.50) below alignment show class F Ballesteros–Weinstein nomenclature for GPCR helices16. Residues interacting with cholesterol are highlighted in red. Disulfide bridges are highlighted in yellow and numbered. N-linked glycosylation sites are depicted by a hexagon. The position of the Val329Phe point mutation is highlighted in purple.
- Extended Data Figure 2: Characterization of the SMO Val329Phe mutation. (319 KB)
a, Superposition of SMOΔC structure (blue) with the SMO-SANT-1 complex structure, which lacks the CRD (green, PDB 4N4W (ref. 16)), showing the TMD ligand-binding pocket as a yellow surface. Inset shows Val329, mutated to Phe in our structure. b, SEC analysis of fluorescently labelled SMOΔC showing difference in expression levels of wild-type and Val329Phe variant (main protein peak ~20 min). c, 20(S)-OHC beads can bind both mouse wild-type SMO and Val333Phe (mouse Val333 corresponds to human Val329). Immunoblots, using an anti-SMO antibody directed against the ICD, were used to measure SMO captured on 20(S)-OHC beads. Adding 50 μM free 20(S)-OHC as a competitor reduced binding. d, Purified human SMOΔC (the crystallization construct) binds to 20(S)-OHC beads. e, Smo−/− mouse fibroblasts stably expressing SMO-WT or SMO-Val333Phe were exposed to SHH, SAG or 20(S)-OHC. Levels of endogenous Gli1 mRNA (mean arbitrary units ± s.d., n = 4), measured by qRT–PCR, were used as a metric of Hh pathway activity because Gli1 is a direct Hh target. Asterisks indicate statistical significance (****P ≤ 0.0001) based on one-way ANOVA for the difference in Gli1 mRNA levels between identically treated SMO-WT and SMO-Val333Phe cells. f, Immunoblot shows SMO and GLI1 protein levels in these stable cell lines, with p38 as loading control. Each experiment was replicated ≥2 times with similar results.
- Extended Data Figure 3: Crystallization, structure solution and oligomeric state of SMOΔC. (741 KB)
a, SMOΔC crystal packing. Asymmetric unit consists of two antiparallel SMOΔC chains. Chain A coloured as in Fig. 1 with BRIL fusion in yellow; Chain B in grey. LCP crystal packing with alternating hydrophobic and hydrophilic layers perpendicular to the c axis. Molecules coloured as for Chain A. b, Pearson correlation coefficient (CC) analysis86 used to relate data quality with model quality. A CCwork and CCfree smaller than CC* indicates that the model does not account for all of the signal in the data (and is therefore not overfit). c–e, SigmaA-weighted 2Fo–Fc electron density maps of final refinement at 1.0σ contour level. c, Val329Phe mutation. d, Extra density within TMD ligand-binding pocket (Fo–Fc maps shown at contour level of +3σ (green) and –3σ (red)) (This density could not be confidently assigned, probably because of low occupancy within the crystal.). e, ‘Connector’ region linking the CRD and linker domain, with Asn188 and linked N-acetyl glycosamine moiety. f, SEC–MALS analysis of amphipol-solubilized SMOΔC. Molar masses (MW, black lines) and 280 nm absorption (grey line) plotted against elution time. MW derived from protein-conjugate analysis indicated in parentheses. For clarity, graphs of MW are shown only around main absorption peak. Theoretical MW of SMOΔC based on sequence is 71 kDa with the extra mass observed in MALS (~13 kDa) probably due to three N-linked glycosylation sites. Expected mass of protein-bound amphipol (A8–35) was previously determined to be 40–75 kDa (ref. 87), in agreement with our data. This analysis suggests that SMOΔC is a monomer under our purification conditions.
- Extended Data Figure 4: Interfaces of the SMO CRD. (556 KB)
a, b, Detailed interactions of the CRD with the connector region (a) and with the linker domain–TMD segment (b). The number of interactions are indicated in the top panel and coloured as indicated in the key box. For non-bonded contacts, the width of the striped line is proportional to the number of atomic contacts. Residue colouring is according to amino acid: blue, positive (H,K,R); red, negative (D,E); green, neutral (S,T,N,Q); grey, aliphatic (A,V,L,I,M); purple, aromatic (F,Y,W); orange, proline (P) or glycine (G); yellow, cysteine (C). The figure is adopted from the PDBSUM server (http://www.ebi.ac.uk/pdbsum/).
- Extended Data Figure 5: Comparison of SMOΔC with previously determined SMO TMD structures. (473 KB)
Superposition of the SMOΔC structure with SMO TMD structures. Structural alignment was performed using the 7TM bundle as template (not including the linker domain or TMD helix 8). SMOΔC (red), SMO TMD complexed with cyclopamine (light orange, PDB 4O9R, r.m.s.d. 0.598 Å for 243 equivalent Cα positions), antaXV (light blue, PDB 4QIM, r.m.s.d. 0.515 Å for 233 equivalent Cα positions), SANT1 (pale cyan, PDB 4N4W, r.m.s.d. 0.483 Å for 240 equivalent Cα positions), LY2940680 (pale green, PDB 4JKV, r.m.s.d. 0.493 Å for 230 equivalent Cα positions), SAG1.5 (pale yellow, PDB 4QIN, r.m.s.d. 0.623 Å for 262 equivalent Cα positions). The box shows a close-up view of the linker domain region revealing a structural rearrangement in the SMOΔC structure compared to the previously determined SMO TMD structures lacking the native extracellular domain.
- Extended Data Figure 6: Cholesterol stabilizes SMO. (1,208 KB)
a–e, MD simulations of SMO in a lipid bilayer. a, SMO embedded in a lipid bilayer with the CRD in orange, the seven-pass transmembrane region excluding intra- and extracellular loops (7TM) in blue and cholesterol in cyan. b–d, Relative r.m.s. fluctuations of the Cα atoms over the course of 5 × 100 ns of atomistic MD simulation in the presence and absence of cholesterol. The structures in b and c are shown as putty representations coloured from high conformational stability (that is, low r.m.s. fluctuations; blue/thin) to low stability (that is, high r.m.s. fluctuations; red/thick). e, Secondary structure DSSP matrices for each of the simulations. The asterisks in b, c and e all mark the helix spanning residues 155–160, which is destabilized in the absence of bound cholesterol. f, g, Thermostability of purified SMOΔC. See Supplementary Discussion for details. f, Compiled peak heights from thermostability SEC analysis of purified SMOΔC after treatment with different MBCD concentrations. g, Example of raw SEC data used for the analysis in f. Samples were incubated at 35 °C for 1 h before loading onto the SEC column.
- Extended Data Figure 7: Effect of domain interface mutations on expression levels and 20(S)-OHC binding. (187 KB)
a, Protein levels of SMO and also of PTCH1 and GLI1 (each of which is encoded by a direct Hh target gene) measured by immunoblot from Smo−/− mouse fibroblasts stably expressing one of five SMO variants: wild-type SMO (WT); SMO lacking the entire CRD (ΔCRD); SMO with two mutations (Pro120Ser or Ile160Asn/Glu162Thr) that introduce glycosylation sites in the linker domain–CRD interface; and SMO lacking a conserved disulfide bond (Cys197Ser/Cys217Ser, marked 6 in Fig. 4c) in the linker domain. Elevated levels of GLI1 and PTCH1 reflect high constitutive signalling activity of each mutant. NS, a non-specific band detected by anti-PTCH1 antibody; SUFU, loading control. Different patterns seen in SMO panel are caused by different numbers of N-linked glycosylation sites. b, Gli1 mRNA levels (mean arbitrary units ± s.d., n = 3) were used to assess Hh signalling activity in Smo−/− cells stably expressing the indicated mouse SMO variants. One-way ANOVA was used to assess statistical significance (****P ≤ 0.0001). D477R and M2 (Trp539Leu) are two previously described mutations in the TMD that increase constitutive signalling. c, Oxysterol-binding capacity of each SMO variant was determined (right blot) by its ability to bind to 20(S)-OHC beads in the absence or presence of 50 μM free 20(S)-OHC. Inputs for each binding reaction are shown on the left. Each experiment was repeated 2 or more times with similar results.
- Extended Data Figure 8: SAXS analysis of SMOΔC. (254 KB)
a, Overlay of size-exclusion chromatograms monitored at 280 nm (A280) collected during SAXS measurements for apo-SMOΔC (red), (+)20(S)-OHC SMOΔC (blue), amphipol (green) and BSA standard (black). Amphipol and BSA were injected at 10 mg ml−1. Inset shows curves normalized to peak height. BSA was used as a reference with a radius-of-hydration of 3.7 nm. Absorbance of the free amphipol is negligible and elutes ~5 min after the amphipol-stabilized SMOΔC samples. b, Dimensionless Kratky plot of apo- and (+)20(S)-OHC-loaded SMOΔC SAXS data. Cross-hairs denote the Guinier–Kratky point (√3, 1.1), the peak position for an ideal, globular particle. The slower decay of the transformed scattering intensities for (+)20(S)-OHC (blue) indicate a comparably less spherical particle.
- Extended Data Figure 9: Crystal structure of the SMOΔC–vismodegib complex and structural analysis of mutations found in vismodegib-resistant cancers. (601 KB)
a, Chemical structure of vismodegib. b, Close-up view of vismodegib-binding site. Colour-coding follows Fig. 5b. Composite omit map calculated with PHENIX at 1.0σ shown as magenta chicken-wire. c, Mapping of residues that are mutated in vismodegib-resistant tumours (yellow highlights). Brackets indicate mutant residues. d–f, Close-up views of selected interactions. Native residues in blue and mutated residues in yellow. Arrows indicate position of potential clashes that could disrupt vismodegib binding. d, Gln477/Asp473 hot spot. The Gln477Glu mutation leads to a loss of the potential hydrogen bond of the glutamine sidechain to the chloride of the vismodegib chlorophenyl-methylsulfone moiety. The Asp473His mutation potentially destabilizes the hydrogen-bonding network around Arg400 that coordinates the vismodegib chlorophenyl-methylsulfone moiety. e, The imidazole ring of His231 is within hydrogen-bonding distance of two carbonyl main-chain atoms of residues Ser385 and Val386 located on a loop coordinating the interaction of Asp384 with vismodegib’s amide linker. f, Trp281 forms a key hydrophobic interaction with the vismodegib pyrimidine ring that is deeply buried in the SMO helical bundle core. Mutation to cysteine would significantly destabilize this interaction while mutation of nearby Val321 to the bulkier methionine would probably result in a rearrangement of the Trp281 side chain. g, h, SMOΔC captured on cholesterol beads in the presence of increasing concentrations of free vismodegib or 20(S)-OHC (h). Results from one of two independent pull-down experiments are shown.
Extended Data Tables
- Supplementary Information (125 KB)
This file contains a Supplementary Discussion and References.