Table of Contents

Volume 520 Number 7547 pp263-400

16 April 2015

About the cover

Cultured cell lines, including many cancer cell lines, are essential tools not only in biological research but also other areas of science. Unfortunately, it has been long known that many cell lines are contaminated, mislabelled or incorrectly annotated. Richard Neve and colleagues now present an analysis providing unambiguous authentication and annotation for more than 3,500 cell lines. This resource offers a reference point for both short tandem repeats (STR) and single nucleotide polymorphism (SNP) profiles as genetic methods of cell annotation, combined with suggested standards for nomenclature using a controlled vocabulary. In a parallel development, we present on page 264 a new publication policy designed to increase the scrutiny around cell line identity problems. Cover: AXS Biomedical Animation Studio

This Week



World View


Seven Days

  • Seven days: 10–16 April 2015

    The week in science: Protests delay telescope construction, Brazil approves GM eucalyptus; and African Centres for Disease Control and Prevention takes shape.

News in Focus


  • Cancer: The Ras renaissance

    No Alt text available for this image

    Thirty years of pursuit have failed to yield a drug to take on one of the deadliest families of cancer-causing proteins. Now some researchers are taking another shot.

    • Heidi Ledford


  • Astronomy: Hubble's legacy

    Twenty-five years after launch, the wild success of the space telescope argues for a new era of bold exploration in the face of tight budgets, says Mario Livio.

Books and Arts

  • Books in brief

    Barbara Kiser reviews five of the week's best science picks.

  • Q&A: Geological historian

    The first geological map of a nation was made 200 years ago by British surveyor William Smith; the rediscovery of a first-edition copy in the archives of the Geological Society of London was announced last month (see As researchers gather for a conference to celebrate the anniversary of the 1815 chart of England and Wales, John Henry, chair of the society's history group, talks about the map and its pioneering creator.

    • Review of William Smith Meeting 2015: 200 Years of Smith's Map








naturejobs job listings and advertising features


  • The buyout

    Share and share alike.

    • Ananyo Bhattacharya


Technology Feature

  • Cancer: A most exceptional response

    Sometimes a drug causes a tumour to completely recede, but only in a tiny percentage of people. Scientists want to decipher such outlier responses for the benefit of all patients.

    • Vivien Marx


Brief Communication Arising



  • A resource for cell line authentication, annotation and quality control

    • Mamie Yu
    • Suresh K. Selvaraj
    • May M. Y. Liang-Chu
    • Sahar Aghajani
    • Matthew Busse
    • Jean Yuan
    • Genee Lee
    • Franklin Peale
    • Christiaan Klijn
    • Richard Bourgon
    • Joshua S. Kaminker
    • Richard M. Neve

    Competing financial interests. The majority of authors are employees of Genentech Inc. and/or hold stock in Roche.


  • Crystal structures of the human adiponectin receptors

    • Hiroaki Tanabe
    • Yoshifumi Fujii
    • Miki Okada-Iwabu
    • Masato Iwabu
    • Yoshihiro Nakamura
    • Toshiaki Hosaka
    • Kanna Motoyama
    • Mariko Ikeda
    • Motoaki Wakiyama
    • Takaho Terada
    • Noboru Ohsawa
    • Masakatsu Hato
    • Satoshi Ogasawara
    • Tomoya Hino
    • Takeshi Murata
    • So Iwata
    • Kunio Hirata
    • Yoshiaki Kawano
    • Masaki Yamamoto
    • Tomomi Kimura-Someya
    • Mikako Shirouzu
    • Toshimasa Yamauchi
    • Takashi Kadowaki
    • Shigeyuki Yokoyama

    The crystal structures of the human adiponectin receptors AdipoR1 and AdipoR2 are solved at 2.9 and 2.4 Å resolution, respectively; the structural and functional information may aid the development and optimization of adiponectin receptor agonists for the treatment of obesity-related diseases.

  • Two disparate ligand-binding sites in the human P2Y1 receptor

    • Dandan Zhang
    • Zhan-Guo Gao
    • Kaihua Zhang
    • Evgeny Kiselev
    • Steven Crane
    • Jiang Wang
    • Silvia Paoletta
    • Cuiying Yi
    • Limin Ma
    • Wenru Zhang
    • Gye Won Han
    • Hong Liu
    • Vadim Cherezov
    • Vsevolod Katritch
    • Hualiang Jiang
    • Raymond C. Stevens
    • Kenneth A. Jacobson
    • Qiang Zhao
    • Beili Wu

    Two X-ray crystal structures are presented of the human P2Y1 G-protein-coupled receptor, which is an important target for anti-thrombotic drugs; the structures unexpectedly reveal two ligand-binding sites.


  • Nuclear ashes and outflow in the eruptive star Nova Vul 1670

    • Tomasz Kamiński
    • Karl M. Menten
    • Romuald Tylenda
    • Marcin Hajduk
    • Nimesh A. Patel
    • Alexander Kraus

    Observations of CK Vulpeculae reveal that it is surrounded by molecular gas and dust whose large mass and chemical composition cannot have come from a nova; the best explanation is that this object is the remnant of the merger of two stars.

  • An ultrafast rechargeable aluminium-ion battery

    • Meng-Chang Lin
    • Ming Gong
    • Bingan Lu
    • Yingpeng Wu
    • Di-Yan Wang
    • Mingyun Guan
    • Michael Angell
    • Changxin Chen
    • Jiang Yang
    • Bing-Joe Hwang
    • Hongjie Dai

    An aluminium-ion battery is reported that can charge within one minute, and offers improved cycle life compared to previous devices; it operates through the electrochemical deposition and dissolution of aluminium at the anode, and the intercalation/de-intercalation of chloroaluminate anions into a novel graphitic-foam cathode.

  • Icebergs not the trigger for North Atlantic cold events

    • Stephen Barker
    • James Chen
    • Xun Gong
    • Lukas Jonkers
    • Gregor Knorr
    • David Thornalley

    A delay between surface cooling and the arrival of ice-rafted debris at a site southwest of Iceland over the past four glacial cycles implies that icebergs typically arrived too late to have triggered cooling, although the freshwater derived from melting icebergs may provide a positive feedback for cold stadial conditions.

  • Recovery potential of the world's coral reef fishes

    • M. Aaron MacNeil
    • Nicholas A. J. Graham
    • Joshua E. Cinner
    • Shaun K. Wilson
    • Ivor D. Williams
    • Joseph Maina
    • Steven Newman
    • Alan M. Friedlander
    • Stacy Jupiter
    • Nicholas V. C. Polunin
    • Tim R. McClanahan

    A study of the recovery potential of over 800 of the world's coral reefs shows that 83% of fished reefs are missing more than half their expected biomass, with severe consequences for key ecosystem functions; protection from fishing would allow full recovery in 35 years on average, but in 59 years for the most degraded reefs.

    See also
  • Emotional learning selectively and retroactively strengthens memories for related events

    • Joseph E. Dunsmoor
    • Vishnu P. Murty
    • Lila Davachi
    • Elizabeth A. Phelps

    Initially weak episodic memories in humans can be selectively enhanced and consolidated following later emotional learning involving conceptually related information, suggesting a mechanism for how we can remember initially inconsequential information after a relevant later experience.

  • The evolutionary history of lethal metastatic prostate cancer

    • Gunes Gundem
    • Peter Van Loo
    • Barbara Kremeyer
    • Ludmil B. Alexandrov
    • Jose M. C. Tubio
    • Elli Papaemmanuil
    • Daniel S. Brewer
    • Heini M. L. Kallio
    • Gunilla Högnäs
    • Matti Annala
    • Kati Kivinummi
    • Victoria Goody
    • Calli Latimer
    • Sarah O'Meara
    • Kevin J. Dawson
    • William Isaacs
    • Michael R. Emmert-Buck
    • Matti Nykter
    • Christopher Foster
    • Zsofia Kote-Jarai
    • Douglas Easton
    • Hayley C. Whitaker
    • ICGC Prostate UK Group
    • David E. Neal
    • Colin S. Cooper
    • Rosalind A. Eeles
    • Tapio Visakorpi
    • Peter J. Campbell
    • Ultan McDermott
    • David C. Wedge
    • G. Steven Bova

    The subclonal composition of human prostate tumours and their metastases has been mapped by whole-genome sequencing, thus establishing the evolutionary trees behind the development and spread of these cancers; an important observation was that metastases could be re-seeded multiple times, and spread from one tumour to another was frequently seen.

    See also
  • A model of breast cancer heterogeneity reveals vascular mimicry as a driver of metastasis

    • Elvin Wagenblast
    • Mar Soto
    • Sara Gutiérrez-Ángel
    • Christina A. Hartl
    • Annika L. Gable
    • Ashley R. Maceli
    • Nicolas Erard
    • Alissa M. Williams
    • Sun Y. Kim
    • Steffen Dickopf
    • J. Chuck Harrell
    • Andrew D. Smith
    • Charles M. Perou
    • John E. Wilkinson
    • Gregory J. Hannon
    • Simon R. V. Knott

    Different clones of a mammary tumour cell line possess differential abilities to contribute to the formation of metastasis; the expression of Serpine2 and Slp1 proteins drives vascular mimicry and metastasis to the lung, with similar associations observed in human data sets, and these proteins also function as anticoagulants, thus further promoting extravasation of tumour cells.

    See also
  • SHMT2 drives glioma cell survival in ischaemia but imposes a dependence on glycine clearance

    • Dohoon Kim
    • Brian P. Fiske
    • Kivanc Birsoy
    • Elizaveta Freinkman
    • Kenjiro Kami
    • Richard L. Possemato
    • Yakov Chudnovsky
    • Michael E. Pacold
    • Walter W. Chen
    • Jason R. Cantor
    • Laura M. Shelton
    • Dan Y. Gui
    • Manjae Kwon
    • Shakti H. Ramkissoon
    • Keith L. Ligon
    • Seong Woo Kang
    • Matija Snuderl
    • Matthew G. Vander Heiden
    • David M. Sabatini

    Tumours are a low-oxygen environment, in this study glioblastoma cells are found to overexpress the serine hydroxymethyltransferase SHMT2; SHMT acts to reduce oxygen consumption, which confers the tumour cells with a survival advantage.

  • Therapy-induced tumour secretomes promote resistance and tumour progression

    • Anna C. Obenauf
    • Yilong Zou
    • Andrew L. Ji
    • Sakari Vanharanta
    • Weiping Shu
    • Hubing Shi
    • Xiangju Kong
    • Marcus C. Bosenberg
    • Thomas Wiesner
    • Neal Rosen
    • Roger S. Lo
    • Joan Massagué

    Tumour cells respond to an effective, targeted drug treatment with BRAF, ALK or EGFR kinase inhibitors by inducing a complex network of secreted signals that promote tumour growth, dissemination and metastasis of drug-resistant cancer cell clones, and increase the survival of drug-sensitive tumour cells, potentially contributing to incomplete tumour regression.

  • Radiation and dual checkpoint blockade activate non-redundant immune mechanisms in cancer

    • Christina Twyman-Saint Victor
    • Andrew J. Rech
    • Amit Maity
    • Ramesh Rengan
    • Kristen E. Pauken
    • Erietta Stelekati
    • Joseph L. Benci
    • Bihui Xu
    • Hannah Dada
    • Pamela M. Odorizzi
    • Ramin S. Herati
    • Kathleen D. Mansfield
    • Dana Patsch
    • Ravi K. Amaravadi
    • Lynn M. Schuchter
    • Hemant Ishwaran
    • Rosemarie Mick
    • Daniel A. Pryma
    • Xiaowei Xu
    • Michael D. Feldman
    • Tara C. Gangadhar
    • Stephen M. Hahn
    • E. John Wherry
    • Robert H. Vonderheide
    • Andy J. Minn

    In this study, involving melanoma patients and a mouse model for melanoma, an optimal anti-tumour response was induced by using a combination of radiation with anti-CTLA4 and anti-PD-L1 antibody therapies, each attacking the tumour from a different angle.

  • Theileria parasites secrete a prolyl isomerase to maintain host leukocyte transformation

    • J. Marsolier
    • M. Perichon
    • J. D. DeBarry
    • B. O. Villoutreix
    • J. Chluba
    • T. Lopez
    • C. Garrido
    • X. Z. Zhou
    • K. P. Lu
    • L. Fritsch
    • S. Ait-Si-Ali
    • M. Mhadhbi
    • S. Medjkane
    • J. B. Weitzman

    Parasites of the Theileria genus infect cattle and transform their host cells, a transformation that can be reversed by treatment with the drug buparvaquone; here, a Theileria homologue of the peptidyl-prolyl isomerase PIN1 is shown to be secreted into the host cell, where it promotes transformation and can be directly inhibited by buparvaquone.


  • Corrigendum: Human gut Bacteroidetes can utilize yeast mannan through a selfish mechanism

    • Fiona Cuskin
    • Elisabeth C. Lowe
    • Max J. Temple
    • Yanping Zhu
    • Elizabeth A. Cameron
    • Nicholas A. Pudlo
    • Nathan T. Porter
    • Karthik Urs
    • Andrew J. Thompson
    • Alan Cartmell
    • Artur Rogowski
    • Brian S. Hamilton
    • Rui Chen
    • Thomas J. Tolbert
    • Kathleen Piens
    • Debby Bracke
    • Wouter Vervecken
    • Zalihe Hakki
    • Gaetano Speciale
    • Jose L. Munōz-Munōz
    • Andrew Day
    • Maria J. Peña
    • Richard McLean
    • Michael D. Suits
    • Alisdair B. Boraston
    • Todd Atherly
    • Cherie J. Ziemer
    • Spencer J. Williams
    • Gideon J. Davies
    • D. Wade Abbott
    • Eric C. Martens
    • Harry J. Gilbert


  • Erratum: A new antibiotic kills pathogens without detectable resistance

    • Losee L. Ling
    • Tanja Schneider
    • Aaron J. Peoples
    • Amy L. Spoering
    • Ina Engels
    • Brian P. Conlon
    • Anna Mueller
    • Till F. Schäberle
    • Dallas E. Hughes
    • Slava Epstein
    • Michael Jones
    • Linos Lazarides
    • Victoria A. Steadman
    • Douglas R. Cohen
    • Cintia R. Felix
    • K. Ashley Fetterman
    • William P. Millett
    • Anthony G. Nitti
    • Ashley M. Zullo
    • Chao Chen
    • Kim Lewis