Identification of a mast-cell-specific receptor crucial for pseudo-allergic drug reactions

Journal name:
Nature
Volume:
519,
Pages:
237–241
Date published:
DOI:
doi:10.1038/nature14022
Received
Accepted
Published online

Mast cells are primary effectors in allergic reactions, and may have important roles in disease by secreting histamine and various inflammatory and immunomodulatory substances1, 2. Although they are classically activated by immunoglobulin (Ig)E antibodies, a unique property of mast cells is their antibody-independent responsiveness to a range of cationic substances, collectively called basic secretagogues, including inflammatory peptides and drugs associated with allergic-type reactions1, 3. The pathogenic roles of these substances have prompted a decades-long search for their receptor(s). Here we report that basic secretagogues activate mouse mast cells in vitro and in vivo through a single receptor, Mrgprb2, the orthologue of the human G-protein-coupled receptor MRGPRX2. Secretagogue-induced histamine release, inflammation and airway contraction are abolished in Mrgprb2-null mutant mice. Furthermore, we show that most classes of US Food and Drug Administration (FDA)-approved peptidergic drugs associated with allergic-type injection-site reactions also activate Mrgprb2 and MRGPRX2, and that injection-site inflammation is absent in mutant mice. Finally, we determine that Mrgprb2 and MRGPRX2 are targets of many small-molecule drugs associated with systemic pseudo-allergic, or anaphylactoid, reactions; we show that drug-induced symptoms of anaphylactoid responses are significantly reduced in knockout mice; and we identify a common chemical motif in several of these molecules that may help predict side effects of other compounds. These discoveries introduce a mouse model to study mast cell activation by basic secretagogues and identify MRGPRX2 as a potential therapeutic target to reduce a subset of drug-induced adverse effects.

At a glance

Figures

  1. Mrgprb2 is the orthologue of human MRGPRX2.
    Figure 1: Mrgprb2 is the orthologue of human MRGPRX2.

    a, Diagram of mouse and human Mrgpr genomic loci. Mouse Mrgpra3 and Mrgprc11 are orthologues of human MRGPRX1, determined by expression and ligand specificity15. The MRGPRX2 orthologue Mrgprb2 is described in this study. Chr., chromosome. b, Results from a stringent RT–PCR screen identifying Mrgprb2 transcript (arrow) in mouse peritoneal mast cells. The negative control (Neg.) omitted reverse transcriptase. RT–PCR for Mrgprb2 was repeated at least four times. c, Example traces of intracellular calcium concentrations [Ca2+]i, measured by ratiometric Fura-2 imaging, from Mrgprb2-HEK or MRGPRX2-HEK cells exposed to 20 µM PAMP(9–20) (duration indicated by black line). Each trace is a response from a unique cell. d, Representative confocal images from BAC transgenic mouse tissues in which tdTomato expression is controlled by enhanced green fluorescent protein (eGFP)-Cre expression from the Mrgprb2 locus (see Methods). Avidin staining was used to identify mast cells. Percentages of avidin-positive mast cells that were also tdTomato positive: glabrous skin, 97.5%; hairy skin, 90.1%; trachea, 97.2%; heart, 87.1%. Percentages of tdTomato-positive cells that were also avidin positive: glabrous skin, 99.2%; hairy skin, 100%; trachea, 98.3%; heart, 99%. n = 3 mice and >300 cells counted per tissue, except n = 2 and >100 cells counted in the heart. Scale bar, 20 µm.

  2. Mrgprb2 is the mouse mast cell basic secretagogue receptor.
    Figure 2: Mrgprb2 is the mouse mast cell basic secretagogue receptor.

    a, Left, representative Fluo-4 fluorescence heat map images of mouse peritoneal mast cells showing changes in [Ca2+]i induced by bath application of anti-IgE (5 µg ml−1) or 48/80 (10 µg ml−1). Middle, representative imaging traces. Each colour line represents an individual cell. Black lines in ‘anti-IgE’ panels are average traces for each genotype. Note that [Ca2+]i traces are similar between wild-type (WT) and mutant (MUT) groups. Right, quantification of responding cells (n = 3 per genotype; >150 cells counted per condition). Anti-IgE responses were not significantly different. Scale bar, 10 µm. b, Histamine release into the supernatant from trachea and abdominal skin from wild-type and Mrgprb2MUT mice after exposure to 48/80 (30 µg ml−1) for 30 min at 37 °C. n = 5 for trachea, n = 8 for skin. c, Top, representative traces showing contractions of trachea isolated from wild-type and Mrgprb2MUT mice (previously sensitized to ovalbumin (Ova)) in response to 48/80 (30 µg ml−1) or ovalbumin (10 µg ml−1; that is, IgE dependent). Bottom, average data; maximum total contraction determined as response to 10 µM carbamycholine added at the end of the experiment. n = 5 for 48/80 wild type, n = 3 for 48/80 MrgprB2MUT. d, Left, representative images of Evans blue stained extravasation 15 min after intraplantar injection of 48/80 (right, arrow, 10 µg ml−1, 5 µl in saline) or saline (left). Right, quantification of Evans blue leakage into the paw and paw thickness increase after 15 min. OD620 nm, optical density at 620 nm. *P < 0.02 (n = 5 wild type, n = 6 MrgprB2MUT). Differences after saline injection were not significant. e, Quantification of wild-type and Mrgprb2MUT mast cell responsiveness to MRGPRX2 ligands and basic secretagogues, assayed using Fluo-4 imaging. Concentrations of substances (in µM): PAMP(9–20), 20; cortistatin-14 (Cort.), 20; substance P (Sub P), 200; kallidin, 200; mastoparan (masto.; a component of wasp venom), 20; vespid mastoparan, 20. n = 3 per genotype; >150 cells counted per secretagogue. Data are presented as mean ± standard error of the mean (s.e.m.). Two-tailed unpaired Student’s t-test was used to determine significance in statistical comparisons, and differences were considered significant at P < 0.05. *P < 0.05, **P < 0.01.

  3. Mrgprb2 mediates mast cell responsiveness and side effects of peptidergic therapeutic drugs.
    Figure 3: Mrgprb2 mediates mast cell responsiveness and side effects of peptidergic therapeutic drugs.

    a, Percentage of responding cells from wild-type (WT) and Mrgprb2MUT (MUT) peritoneal mast cells after drug application, assayed using Fluo-4 imaging. Concentrations of drugs (in µg ml−1): icatibant, 50; cetrorelix, 20; leuprolide, 100; octreotide, 10; sermorelin, 60; insulin, 80. n = 3 per genotype; >150 cells counted per substance, except >100 cells counted for insulin. Difference between insulin responsiveness was not significant. b, Left, representative images of Evans blue stained extravasation 15 min after intraplantar injection of icatibant (right, arrow, 10 mg ml−1, 5 µl in saline) or saline (left). Right, quantification of Evans blue leakage into the paw after 15 min. n = 6 per genotype. Difference after saline injection was not significant. c, Total histamine release from wild-type (red diamonds) and Mrgprb2MUT (black squares) mice after incubation with named substances. Note that no significant difference between wild-type and Mrgprb2MUT cells was found at any dose of anti-IgE antibody. Experiments were repeated >3 times. Data are presented as mean ± s.e.m. Two-tailed unpaired Student’s t-test: *P < 0.05, **P < 0.01. NS, not significant.

  4. Mrgprb2 mediates mast cell responsiveness and side effects of small-molecule therapeutic drugs.
    Figure 4: Mrgprb2 mediates mast cell responsiveness and side effects of small-molecule therapeutic drugs.

    a, Structures of 48/80 and a cyclized variant. The THIQ motif is highlighted in blue. b, Structures of representative members of all NMBD classes (see Supplementary Information). THIQ motifs are highlighted in blue. Note that only succinylcholine lacks a bulky hydrophobic group. c, Percentage of responding cells from wild-type (WT) and Mrgprb2MUT (MUT) peritoneal mast cells after application of various NMBDs, assayed using Fluo-4 imaging. Concentrations of drugs (in µg ml−1): atracurium, 50; mivacurium, 20; tubocurarine, 30; rocuronium, 500. n = 3 mice per genotype; >150 cells counted per substance. d, Structure of ciprofloxacin, with the motif common to all fluoroquinolones highlighted in blue. Note the nitrogens close to the quinolone motif. e, Percentage of responding cells from wild-type and Mrgprb2MUT peritoneal mast cells after fluoroquinolone application, assayed using Fluo-4 imaging. Concentrations of drugs (in µg ml−1): ciprofloxacin, 200; levofloxacin, 500; moxifloxacin, 160; ofloxacin, 400. n = 3 mice per genotype; >150 cells counted per substance. f, Changes in body temperature after intravenous injection of ciprofloxacin (1.5 mg in 125 µl saline) at time 0. n = 4 mice per genotype. Data are presented as mean ± s.e.m. Two-tailed unpaired Student’s t-test: *P < 0.05, **P < 0.01.

  5. MRGPRX1 orthologues are not expressed at relevant levels in mast cells under naive conditions.
    Extended Data Fig. 1: MRGPRX1 orthologues are not expressed at relevant levels in mast cells under naive conditions.

    a, Results from a low-stringency RT–PCR screen (see Methods) in peritoneal mast cells for expression of the MRGPRX1 orthologues Mrgpra3 and Mrgprc11. Arrow points to expected band sizes. b, Percentages of peritoneal mast cells responding to the MRGPRX1 and Mrgprc11 agonist bovine adrenal medulla derived peptide, fragment 8–22 (BAM8–22, 500 nM). Activation was assayed by measuring rises in intracellular calcium, using imaging of the Fluo-4 dye. Differences are not significant (P = 0.39). n = 3 mice from each genotype. Group data are expressed as mean ± s.e.m. Two-tailed unpaired Student’s t-test was used to determine significance in statistical comparisons. WT, wild type; KO, knockout. c, Chart summarizing responses to MRGPRX2 ligands and the MRGPRX1 ligand chloroquine (CQ) by HEK293 cells transiently transfected with plasmids driving expression of MRGPRX2, Mrgprb2 and other mouse Mrgpr proteins (that is, Mrgprb1, Mrgprb10 and Mrgprb11) most closely related to Mrgprb2. Positive and negative responses are indicated with ticks and crosses, respectively. Responses were considered positive if at least half of the transfected cells showed a 50% increase in [Ca2+]i. No cells transfected with Mrgprb1, Mrgprb10 and Mrgprb11 responded to any listed drug.

  6. Basic secretagogues and drugs that induce pseudo-allergic reactions activate mouse Mrgprb2 and human MRGPRX2 expressed in HEK293 cells.
    Extended Data Fig. 2: Basic secretagogues and drugs that induce pseudo-allergic reactions activate mouse Mrgprb2 and human MRGPRX2 expressed in HEK293 cells.

    a, b, Example traces showing changes in [Ca2+]i, as measured by Fluo-4 imaging, from HEK293 cells expressing Mrgprb2 and Gα15 (a) or MRGPRX2 and Gα15 (b). Substances were perfused from the 30 to 90 s time period, except for ciprofloxacin, which was perfused between the 30 and 60 s time periods to minimize exposure to the low pH solutions it was dissolved in. Insulin was used as a negative control. c, Table of half-maximum effective concentration (EC50) values of basic secretagogues and drugs associated with pseudo-allergic reactions to activate Mrgprb2- and MRGPRX2-expressing HEK293 cells. The EC50 values were determined from dose–response studies, which were repeated three times. Data are expressed as mean ± s.e.m.

  7. Multiple lines of BAC transgenic mice confirm mast-cell-specific MrgprB2 expression.
    Extended Data Fig. 3: Multiple lines of BAC transgenic mice confirm mast-cell-specific MrgprB2 expression.

    Representative confocal images from two other BAC transgenic mouse lines. BAC mice expressing eGFP-Cre in the Mrgprb2 open reading frame were mated to tdTomato reporter mice and tdTomato (red) expression was compared to avidin staining (green), a marker for mast cells. Scale bar, 20 µm.

  8. Mrgprb2 is not expressed in mucosal mast cells or peripheral white blood cells.
    Extended Data Fig. 4: Mrgprb2 is not expressed in mucosal mast cells or peripheral white blood cells.

    a, Representative images of a stomach section from an Mrgprb2-tdTomato mouse stained with an anti-MCPT1 (β-chymase) antibody to label mucosal mast cells. White arrows indicate positive cells. No cells were double-labelled (296 Mcpt1-labelled cells and 275 tdTomato-positive cells counted, n = 3 mice). Scale bar, 40 µm. b, Representative images of a Cytospin preparation of peripheral white blood cells from an Mrgprb2-tdTomato mouse doubly labelled with tdTomato for Mrgprb2-expressing cells (red; left image) and Hoechst 33342 nuclear staining (blue; right image). No peripheral white blood cell expressed tdTomato (n = 3 mice; >4,000 cells examined). Scale bar, 40 µm.

  9. Mrgprb2MUT mice are functional knockouts.
    Extended Data Fig. 5: Mrgprb2MUT mice are functional knockouts.

    a, Illustration of the genomic region in and around the Mrgprb2 locus. Note that repetitive sequences including long interspersed elements (LINEs), short interspersed elements (SINEs), and long tandem repeats (LTRs) begin immediately after the 3′ side of the Mrgprb2 gene, and in addition are present within 2.5 kb of the 5′ side. A BLASTN search in March 2014 using the 500 bases adjacent to the 3′ end of Mrgprb2 as a query turned up more than 269,000 hits in the mouse genome. b, Comparison of the wild-type (WT) and mutant (MUT) genomic sequences shows the location of the 4 bp deletion in the mutant. Numbers correspond to the Mrgprb2 open reading frame. c, Sequencing result from wild-type and mutant complementary DNA sampled from mice born 18 months after the mutant line was established. The bases missing in the mutant are highlighted in red. d, Amino acid translation of the Mrgprb2MUT open reading frame reveals that the deletion creates a frameshift mutation and an early termination codon (marked with an asterisk) shortly after the first transmembrane region. Mut, site of the frameshift deletion; TM1, transmembrane region 1.

  10. The mast cell numbers and the histamine content of tracheal and skin tissue was not different between wild-type and Mrgprb2MUT animals.
    Extended Data Fig. 6: The mast cell numbers and the histamine content of tracheal and skin tissue was not different between wild-type and Mrgprb2MUT animals.

    a, Top, representative pictures of avidin staining in wild-type (WT) and Mrgprb2MUT (MUT) mice. Scale bar, 40 µm. Bottom, quantification of mast cell numbers in various tissues. Differences are not significant, using a two-tailed unpaired Student’s t-test (n = 3 mice for each genotype; over 3,000 µm2 and 1,000 µm2 counted for each genotype for hairy and glabrous skin, respectively; over 10,000 peritoneal cells counted). b, The tracheal histamine content averaged 5.9 ± 0.9 and 5.5 ± 1.6 ng mg−1 (n = 5 for each genotype), respectively; the skin histamine content averaged 30.8 ± 3.2 and 30.2 ± 4.0 ng mg−1 (n = 8 for each genotype), respectively. Differences were not significant. Group data are expressed as mean ± s.e.m. Two-tailed unpaired Student’s t-test was used to determine significance in statistical comparisons.

  11. Endothelin acting through the ETA GPCR induced comparable activation in Mrgprb2MUT and wild-type mast cells.
    Extended Data Fig. 7: Endothelin acting through the ETA GPCR34 induced comparable activation in Mrgprb2MUT and wild-type mast cells.

    a, Representative heat map images of mouse peritoneal mast cells showing changes in [Ca2+]i, as assayed by Fluo-4 imaging, induced by bath application of endothelin (1 µM). Scale bar, 10 µm. b, Averages of [Ca2+]i imaging traces for wild-type (WT) (red line) and Mrgprb2MUT (MUT) (black line). The [Ca2+]i traces are similar between wild-type and mutant groups. Traces were averaged as described for Fig. 2a. c, Quantification of percentage of responding cells. Group data are expressed as mean ± s.e.m. Two-tailed unpaired Student’s t-test was used to determine significance in statistical comparisons (n = 3 for each genotype; over 180 cells counted for each genotype). Endothelin-induced responses were not significantly different.

  12. IgE-mediated inflammation does not differ between wild-type and MrgprB2MUT mice.
    Extended Data Fig. 8: IgE-mediated inflammation does not differ between wild-type and MrgprB2MUT mice.

    a, Representative images of Evans blue stained extravasation 15 min after intraplantar injection of anti-IgE antibody (right, arrow, 100 µg ml−1, 7 µl in saline) or saline (left). b, Quantification of Evans blue leakage into the paw after 15 min (n = 6 for wild type (WT), n = 7 for MrgprB2MUT (MUT)). Differences after anti-IgE antibody (P = 0.49) and saline (P = 0.23) injection are not significant. Group data are expressed as mean ± s.e.m. Two-tailed unpaired Student’s t-test was used to determine significance in statistical comparisons.

  13. Mrgprb2MUT mast cells are unresponsive to basic secretagogues and various therapeutic drugs.
    Extended Data Fig. 9: Mrgprb2MUT mast cells are unresponsive to basic secretagogues and various therapeutic drugs.

    a, Example traces showing changes in [Ca2+]i, as measured by Fluo-4 imaging, from wild-type (WT) and Mrgprb2MUT (MUT) peritoneal mast cells induced by the basic secretagogues from Fig. 2e. Each trace is a response from a unique cell. b, Representative Fluo-4 images (left) and fluorescence traces (right) from wild-type (top) and Mrgprb2MUT (bottom) cultured peritoneal mast cells during application of icatibant (50 µg ml−1). c, Example traces showing changes in [Ca2+]i, as measured by Fluo-4 imaging, from wild-type and Mrgprb2MUT peritoneal mast cells induced by selected FDA-approved cationic peptidergic drugs. Each trace is a response from a unique cell. d, Representative Fluo-4 images (left) and fluorescence traces (right) from wild-type (top) and Mrgprb2MUT (bottom) cultured peritoneal mast cells during application of atracurium (50 µg ml−1). e, Representative Fluo-4 images (left) and fluorescence traces (right) from wild-type (top) and Mrgprb2MUT (bottom) cultured peritoneal mast cells during application of ciprofloxacin (200 µg ml−1).

  14. Human mast cells are activated by basic secretagogues and drugs associated with pseudo-allergic reactions in an MRGPRX2-dependent manner.
    Extended Data Fig. 10: Human mast cells are activated by basic secretagogues and drugs associated with pseudo-allergic reactions in an MRGPRX2-dependent manner.

    a, Human LAD2 mast cells were treated with different concentrations of compound 48/80, mastoparan, icatibant, atracurium and ciprofloxacin. The activation of mast cells in response to these substances was characterized by the release of β-hexosaminidase, TNF, PGD2 and histamine. In addition, 0.1 µg ml−1 streptavidin stimulation of biotin-conjugated human IgE-sensitized LAD2 cells caused a robust release of β-hexosaminidase (71.3 ± 1.8% release), compared with untreated cells (4.1 ± 0.3% release). Group data are expressed as mean ± s.e.m. b, Knockdown of human MRGPRX2 significantly reduced mast cell activation evoked by basic secretagogues and drugs associated with pseudo-allergic reactions, but not by IgE. Human LAD2 mast cells were first transfected with siRNA against MRGPRX2 or control siRNA. Two days after the transfection, the cells were treated with compound 48/80 (0.1 µg ml−1), mastoparan (5 µg ml−1), icatibant (10 µg ml−1), atracurium (25 µg ml−1) and ciprofloxacin (75 µg ml−1). The activation of mast cells in response to these substances characterized by the release of β-hexosaminidase was significantly reduced in MRGPRX2-siRNA-treated cells, compared to release in the control group. IgE-mediated mast cell degranulation was unaffected by MRGPRX2 siRNA knockdown. Group data are expressed as mean ± s.e.m. Two-tailed unpaired Student’s t-test was used to determine significance in statistical comparisons, and differences were considered significant at *P < 0.05, **P < 0.01, ***P < 0.005 (the experiments were repeated three times).

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Author information

Affiliations

  1. The Solomon H. Snyder Department of Neuroscience, Department of Neurosurgery, Center for Sensory Biology, Johns Hopkins University, School of Medicine, Baltimore, Maryland 21205, USA

    • Benjamin D. McNeil,
    • Liang Han &
    • Xinzhong Dong
  2. Department of Medical Microbiology and Immunology, University of Alberta, Edmonton, Alberta T6G 2E1, Canada

    • Priyanka Pundir &
    • Marianna Kulka
  3. Department of Medicine, Division of Allergy and Clinical Immunology, Johns Hopkins University, School of Medicine, Baltimore, Maryland 21205, USA

    • Sonya Meeker &
    • Bradley J. Undem
  4. National Institute for Nanotechnology, National Research Council Canada, Edmonton, Alberta T6G 2M9, Canada

    • Marianna Kulka
  5. Howard Hughes Medical Institute, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA

    • Xinzhong Dong

Contributions

B.D.M. conceived the project, designed and performed all experiments except where noted, and wrote the paper. P.P. performed all LAD2 cell work with supervision from M.K. S.M. performed tracheal contraction and tissue histamine release experiments. L.H. assisted with BAC purification and staining techniques. B.J.U. supervised S.M. and contributed to experimental design. X.D. supervised the project and wrote the paper.

Competing financial interests

The authors declare no competing financial interests.

Corresponding author

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Extended data figures and tables

Extended Data Figures

  1. Extended Data Figure 1: MRGPRX1 orthologues are not expressed at relevant levels in mast cells under naive conditions. (212 KB)

    a, Results from a low-stringency RT–PCR screen (see Methods) in peritoneal mast cells for expression of the MRGPRX1 orthologues Mrgpra3 and Mrgprc11. Arrow points to expected band sizes. b, Percentages of peritoneal mast cells responding to the MRGPRX1 and Mrgprc11 agonist bovine adrenal medulla derived peptide, fragment 8–22 (BAM8–22, 500 nM). Activation was assayed by measuring rises in intracellular calcium, using imaging of the Fluo-4 dye. Differences are not significant (P = 0.39). n = 3 mice from each genotype. Group data are expressed as mean ± s.e.m. Two-tailed unpaired Student’s t-test was used to determine significance in statistical comparisons. WT, wild type; KO, knockout. c, Chart summarizing responses to MRGPRX2 ligands and the MRGPRX1 ligand chloroquine (CQ) by HEK293 cells transiently transfected with plasmids driving expression of MRGPRX2, Mrgprb2 and other mouse Mrgpr proteins (that is, Mrgprb1, Mrgprb10 and Mrgprb11) most closely related to Mrgprb2. Positive and negative responses are indicated with ticks and crosses, respectively. Responses were considered positive if at least half of the transfected cells showed a 50% increase in [Ca2+]i. No cells transfected with Mrgprb1, Mrgprb10 and Mrgprb11 responded to any listed drug.

  2. Extended Data Figure 2: Basic secretagogues and drugs that induce pseudo-allergic reactions activate mouse Mrgprb2 and human MRGPRX2 expressed in HEK293 cells. (333 KB)

    a, b, Example traces showing changes in [Ca2+]i, as measured by Fluo-4 imaging, from HEK293 cells expressing Mrgprb2 and Gα15 (a) or MRGPRX2 and Gα15 (b). Substances were perfused from the 30 to 90 s time period, except for ciprofloxacin, which was perfused between the 30 and 60 s time periods to minimize exposure to the low pH solutions it was dissolved in. Insulin was used as a negative control. c, Table of half-maximum effective concentration (EC50) values of basic secretagogues and drugs associated with pseudo-allergic reactions to activate Mrgprb2- and MRGPRX2-expressing HEK293 cells. The EC50 values were determined from dose–response studies, which were repeated three times. Data are expressed as mean ± s.e.m.

  3. Extended Data Figure 3: Multiple lines of BAC transgenic mice confirm mast-cell-specific MrgprB2 expression. (339 KB)

    Representative confocal images from two other BAC transgenic mouse lines. BAC mice expressing eGFP-Cre in the Mrgprb2 open reading frame were mated to tdTomato reporter mice and tdTomato (red) expression was compared to avidin staining (green), a marker for mast cells. Scale bar, 20 µm.

  4. Extended Data Figure 4: Mrgprb2 is not expressed in mucosal mast cells or peripheral white blood cells. (509 KB)

    a, Representative images of a stomach section from an Mrgprb2-tdTomato mouse stained with an anti-MCPT1 (β-chymase) antibody to label mucosal mast cells. White arrows indicate positive cells. No cells were double-labelled (296 Mcpt1-labelled cells and 275 tdTomato-positive cells counted, n = 3 mice). Scale bar, 40 µm. b, Representative images of a Cytospin preparation of peripheral white blood cells from an Mrgprb2-tdTomato mouse doubly labelled with tdTomato for Mrgprb2-expressing cells (red; left image) and Hoechst 33342 nuclear staining (blue; right image). No peripheral white blood cell expressed tdTomato (n = 3 mice; >4,000 cells examined). Scale bar, 40 µm.

  5. Extended Data Figure 5: Mrgprb2MUT mice are functional knockouts. (211 KB)

    a, Illustration of the genomic region in and around the Mrgprb2 locus. Note that repetitive sequences including long interspersed elements (LINEs), short interspersed elements (SINEs), and long tandem repeats (LTRs) begin immediately after the 3′ side of the Mrgprb2 gene, and in addition are present within 2.5 kb of the 5′ side. A BLASTN search in March 2014 using the 500 bases adjacent to the 3′ end of Mrgprb2 as a query turned up more than 269,000 hits in the mouse genome. b, Comparison of the wild-type (WT) and mutant (MUT) genomic sequences shows the location of the 4 bp deletion in the mutant. Numbers correspond to the Mrgprb2 open reading frame. c, Sequencing result from wild-type and mutant complementary DNA sampled from mice born 18 months after the mutant line was established. The bases missing in the mutant are highlighted in red. d, Amino acid translation of the Mrgprb2MUT open reading frame reveals that the deletion creates a frameshift mutation and an early termination codon (marked with an asterisk) shortly after the first transmembrane region. Mut, site of the frameshift deletion; TM1, transmembrane region 1.

  6. Extended Data Figure 6: The mast cell numbers and the histamine content of tracheal and skin tissue was not different between wild-type and Mrgprb2MUT animals. (213 KB)

    a, Top, representative pictures of avidin staining in wild-type (WT) and Mrgprb2MUT (MUT) mice. Scale bar, 40 µm. Bottom, quantification of mast cell numbers in various tissues. Differences are not significant, using a two-tailed unpaired Student’s t-test (n = 3 mice for each genotype; over 3,000 µm2 and 1,000 µm2 counted for each genotype for hairy and glabrous skin, respectively; over 10,000 peritoneal cells counted). b, The tracheal histamine content averaged 5.9 ± 0.9 and 5.5 ± 1.6 ng mg−1 (n = 5 for each genotype), respectively; the skin histamine content averaged 30.8 ± 3.2 and 30.2 ± 4.0 ng mg−1 (n = 8 for each genotype), respectively. Differences were not significant. Group data are expressed as mean ± s.e.m. Two-tailed unpaired Student’s t-test was used to determine significance in statistical comparisons.

  7. Extended Data Figure 7: Endothelin acting through the ETA GPCR34 induced comparable activation in Mrgprb2MUT and wild-type mast cells. (117 KB)

    a, Representative heat map images of mouse peritoneal mast cells showing changes in [Ca2+]i, as assayed by Fluo-4 imaging, induced by bath application of endothelin (1 µM). Scale bar, 10 µm. b, Averages of [Ca2+]i imaging traces for wild-type (WT) (red line) and Mrgprb2MUT (MUT) (black line). The [Ca2+]i traces are similar between wild-type and mutant groups. Traces were averaged as described for Fig. 2a. c, Quantification of percentage of responding cells. Group data are expressed as mean ± s.e.m. Two-tailed unpaired Student’s t-test was used to determine significance in statistical comparisons (n = 3 for each genotype; over 180 cells counted for each genotype). Endothelin-induced responses were not significantly different.

  8. Extended Data Figure 8: IgE-mediated inflammation does not differ between wild-type and MrgprB2MUT mice. (172 KB)

    a, Representative images of Evans blue stained extravasation 15 min after intraplantar injection of anti-IgE antibody (right, arrow, 100 µg ml−1, 7 µl in saline) or saline (left). b, Quantification of Evans blue leakage into the paw after 15 min (n = 6 for wild type (WT), n = 7 for MrgprB2MUT (MUT)). Differences after anti-IgE antibody (P = 0.49) and saline (P = 0.23) injection are not significant. Group data are expressed as mean ± s.e.m. Two-tailed unpaired Student’s t-test was used to determine significance in statistical comparisons.

  9. Extended Data Figure 9: Mrgprb2MUT mast cells are unresponsive to basic secretagogues and various therapeutic drugs. (492 KB)

    a, Example traces showing changes in [Ca2+]i, as measured by Fluo-4 imaging, from wild-type (WT) and Mrgprb2MUT (MUT) peritoneal mast cells induced by the basic secretagogues from Fig. 2e. Each trace is a response from a unique cell. b, Representative Fluo-4 images (left) and fluorescence traces (right) from wild-type (top) and Mrgprb2MUT (bottom) cultured peritoneal mast cells during application of icatibant (50 µg ml−1). c, Example traces showing changes in [Ca2+]i, as measured by Fluo-4 imaging, from wild-type and Mrgprb2MUT peritoneal mast cells induced by selected FDA-approved cationic peptidergic drugs. Each trace is a response from a unique cell. d, Representative Fluo-4 images (left) and fluorescence traces (right) from wild-type (top) and Mrgprb2MUT (bottom) cultured peritoneal mast cells during application of atracurium (50 µg ml−1). e, Representative Fluo-4 images (left) and fluorescence traces (right) from wild-type (top) and Mrgprb2MUT (bottom) cultured peritoneal mast cells during application of ciprofloxacin (200 µg ml−1).

  10. Extended Data Figure 10: Human mast cells are activated by basic secretagogues and drugs associated with pseudo-allergic reactions in an MRGPRX2-dependent manner. (248 KB)

    a, Human LAD2 mast cells were treated with different concentrations of compound 48/80, mastoparan, icatibant, atracurium and ciprofloxacin. The activation of mast cells in response to these substances was characterized by the release of β-hexosaminidase, TNF, PGD2 and histamine. In addition, 0.1 µg ml−1 streptavidin stimulation of biotin-conjugated human IgE-sensitized LAD2 cells caused a robust release of β-hexosaminidase (71.3 ± 1.8% release), compared with untreated cells (4.1 ± 0.3% release). Group data are expressed as mean ± s.e.m. b, Knockdown of human MRGPRX2 significantly reduced mast cell activation evoked by basic secretagogues and drugs associated with pseudo-allergic reactions, but not by IgE. Human LAD2 mast cells were first transfected with siRNA against MRGPRX2 or control siRNA. Two days after the transfection, the cells were treated with compound 48/80 (0.1 µg ml−1), mastoparan (5 µg ml−1), icatibant (10 µg ml−1), atracurium (25 µg ml−1) and ciprofloxacin (75 µg ml−1). The activation of mast cells in response to these substances characterized by the release of β-hexosaminidase was significantly reduced in MRGPRX2-siRNA-treated cells, compared to release in the control group. IgE-mediated mast cell degranulation was unaffected by MRGPRX2 siRNA knockdown. Group data are expressed as mean ± s.e.m. Two-tailed unpaired Student’s t-test was used to determine significance in statistical comparisons, and differences were considered significant at *P < 0.05, **P < 0.01, ***P < 0.005 (the experiments were repeated three times).

Supplementary information

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    This file contains a Supplementary Table and Chart and additional references.

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