Streaming video does not reproduce the original video quality. Download the video using the link below to view at original quality.This video shows 320 s of continuous calcium imaging of dendritic spines of GCaMP6s-expressing V1 neurons in response to drifting grating stimulation (2 s duration, 0.05 cycles per degree, 1 Hz temporal frequency). The direction of grating movement is indicated by the arrow in the upper right.
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Janelia Farm Research Campus, Howard Hughes Medical Institute, 19700 Helix Drive, Ashburn, Virginia 20147, USA
- Tsai-Wen Chen,
- Trevor J. Wardill,
- Yi Sun,
- Stefan R. Pulver,
- Amy Baohan,
- Eric R. Schreiter,
- Rex A. Kerr,
- Vivek Jayaraman,
- Loren L. Looger,
- Karel Svoboda &
- Douglas S. Kim
Champalimaud Neuroscience Programme, Champalimaud Centre for the Unknown, Avenida Brasília, Doca de Pedrouços, 1400-038, Lisbon, Portugal
- Sabine L. Renninger &
- Michael B. Orger
Department of Neurobiology, University of California Los Angeles, Los Angeles, California 90095, USA
- Amy Baohan
Present address: Marine Biological Laboratory, Program in Sensory Physiology and Behavior, 7 MBL Street, Woods Hole, Massachusetts 02543, USA.
- Trevor J. Wardill
V.J., R.A.K., L.L.L. and K.S. initiated the project. T.-W.C., T.J.W. and D.S.K. conducted neuronal culture screening. T.-W.C. performed mouse visual cortical experiments on pyramidal neurons. T.-W.C. and A.B. performed experiments on inhibitory neurons. Y.S., T.J.W. and S.R.P. carried out fly larval neuromuscular junction studies. Y.S. and V.J. carried out adult fly antennal lobe imaging. S.L.R. and M.B.O. conducted zebrafish tectal imaging. E.R.S. performed protein assays. All authors analysed data. T.-W.C., R.A.K., M.B.O., V.J., L.L.L., K.S. and D.S.K. wrote the paper with comments from all authors.
Competing financial interests
The authors have applied for a patent on materials and methods related to the GCaMP6 variants.
Video 1: Simultaneous recording of spikes and GCaMP6s fluorescence signal of a L2/3 neuron in the visual cortex in vivo
Video 2: Calcium imaging of dendritic spines of GCaMP6s-expressing V1 neurons in response to drifting grating stimulation