MicroRNAs (miRNAs) show differential expression across breast cancer subtypes, and have both oncogenic and tumour-suppressive roles1, 2, 3, 4, 5, 6. Here we report the miRNA expression profiles of 1,302 breast tumours with matching detailed clinical annotation, long-term follow-up and genomic and messenger RNA expression data7. This provides a comprehensive overview of the quantity, distribution and variation of the miRNA population and provides information on the extent to which genomic, transcriptional and post-transcriptional events contribute to miRNA expression architecture, suggesting an important role for post-transcriptional regulation. The key clinical parameters and cellular pathways related to the miRNA landscape are characterized, revealing context-dependent interactions, for example with regards to cell adhesion and Wnt signalling. Notably, only prognostic miRNA signatures derived from breast tumours devoid of somatic copy-number aberrations (CNA-devoid) are consistently prognostic across several other subtypes and can be validated in external cohorts. We then use a data-driven approach8 to seek the effects of miRNAs associated with differential co-expression of mRNAs, and find that miRNAs act as modulators of mRNA–mRNA interactions rather than as on–off molecular switches. We demonstrate such an important modulatory role for miRNAs in the biology of CNA-devoid breast cancers, a common subtype in which the immune response is prominent. These findings represent a new framework for studying the biology of miRNAs in human breast cancer.
At a glance
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- Supplementary Information (8 MB)
This file contains Supplementary Methods, Supplementary Appendix containing R source code for the modulatory effect of miRNAs, Supplementary References and Supplementary Figures 1-8.
- Supplementary Data (2.9 MB)
This file contains Supplementary Tables 1-9. Supplementary Table 1 contains detailed clinical and histopathological information for the 1,302 tumors; Supplementary Table 2 shows annotation, potential cross-hybridization and distribution of 853 detectable miRNAs; Supplementary Table 3 shows miRNAs regulated by CNAs across the entire cohort or in ER+/ER- sub-cohorts; Supplementary Table 4 contains a list of minimal common regions of CNAs (across the entire cohort or in ER+/ER- sub-cohorts) which contain miRNAs and mRNAs; Supplementary Table 4 contains a list of minimal common regions of CNAs (across the entire cohort or in ER+/ER- sub-cohorts) which contain only miRNAs; Supplementary Table 5 shows intensity and correlation of detectable sibling miRNAs; Supplementary Table 6 contains a list of differentially expressed miRNAs between ER+ and ER- sub-cohorts or between Pam50 subtypes; Supplementary Table 7 shows calculated pairwise generalized additive model values for variable mRNAs and miRNAs; Supplementary Table 8 contains a list of significantly enriched Gene Ontology Biological Process terms in mRNAs clustered by miRNA-mRNA GAM values and Supplementary Table 9 contains a summary of ER and Her2 statuses of the breast cancer cell lines used in this study.