In the cerebral cortex, local circuits consist of tens of thousands of neurons, each of which makes thousands of synaptic connections. Perhaps the biggest impediment to understanding these networks is that we have no wiring diagrams of their interconnections. Even if we had a partial or complete wiring diagram, however, understanding the network would also require information about each neuron's function. Here we show that the relationship between structure and function can be studied in the cortex with a combination of in vivo physiology and network anatomy. We used two-photon calcium imaging to characterize a functional property—the preferred stimulus orientation—of a group of neurons in the mouse primary visual cortex. Large-scale electron microscopy of serial thin sections was then used to trace a portion of these neurons’ local network. Consistent with a prediction from recent physiological experiments, inhibitory interneurons received convergent anatomical input from nearby excitatory neurons with a broad range of preferred orientations, although weak biases could not be rejected.
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- Supplementary Figures (5.1M)
The file contains Supplementary Figures 1-8 with legends.
- Supplementary Movie 1 (30.4M)
The movie shows section-by-section fly-through of the aligned EM series. Please see Methods for directions to the publicly accessible high-resolution aligned dataset.
- Supplementary Movie 2 (30M)
The movie shows section-by-section fly-through of a cropped (8.2 x 8.2 µm) region traversing a sixth of the aligned EM sections at the level of the functionally imaged plane. With global 3-D alignment of the EM data set, many of the finest processes in the neuropil (e.g. dendritic spines and fine axons) can be unambiguously followed for tens to hundreds of micrometres.
- Supplementary Movie 3 (27.7M)
The movie shows fifty serial sections in the aligned EM series rotating as a false-colour volumetric rendering.
- Supplementary Movie 4 (10.3M)
The movie shows zoomed-in view of a region through fifty serial sections rotating as a false-colour volumetric rendering.
- Supplementary Movie 5 (28.7M)
The movie shows rotating 3-D renderings of the skeletonized arbors and cell bodies of the functionally characterized cells, their postsynaptic targets, and their convergence targets.