A three-dimensional model of the yeast genome

Journal name:
Nature
Volume:
465,
Pages:
363–367
Date published:
DOI:
doi:10.1038/nature08973
Received
Accepted
Published online

Layered on top of information conveyed by DNA sequence and chromatin are higher order structures that encompass portions of chromosomes, entire chromosomes, and even whole genomes1, 2, 3. Interphase chromosomes are not positioned randomly within the nucleus, but instead adopt preferred conformations4, 5, 6, 7. Disparate DNA elements co-localize into functionally defined aggregates or ‘factories’ for transcription8 and DNA replication9. In budding yeast, Drosophila and many other eukaryotes, chromosomes adopt a Rabl configuration, with arms extending from centromeres adjacent to the spindle pole body to telomeres that abut the nuclear envelope10, 11, 12. Nonetheless, the topologies and spatial relationships of chromosomes remain poorly understood. Here we developed a method to globally capture intra- and inter-chromosomal interactions, and applied it to generate a map at kilobase resolution of the haploid genome of Saccharomyces cerevisiae. The map recapitulates known features of genome organization, thereby validating the method, and identifies new features. Extensive regional and higher order folding of individual chromosomes is observed. Chromosome XII exhibits a striking conformation that implicates the nucleolus as a formidable barrier to interaction between DNA sequences at either end. Inter-chromosomal contacts are anchored by centromeres and include interactions among transfer RNA genes, among origins of early DNA replication and among sites where chromosomal breakpoints occur. Finally, we constructed a three-dimensional model of the yeast genome. Our findings provide a glimpse of the interface between the form and function of a eukaryotic genome.

At a glance

Figures

  1. Schematic depiction of the method.
    Figure 1: Schematic depiction of the method.

    Our method relies on the 4C procedure by using cross-linking, two rounds of alternating restriction enzyme (RE) digestion (6-bp-cutter RE1 for the 3C-step digestion and 4-bp-cutter RE2 for the 4C-step digestion) and intra-molecular ligation. At step 7, each circle contains the 6-bp restriction enzyme recognition site originally used to link the two interacting partner sequences (RE1). Diverging from 4C, we relinearize the circles using RE1, then sequentially insert two sets of adaptors, one of which permits digestion with a type IIS or type III restriction enzyme (such as EcoP15I). Following EcoP15I digestion, fragments are produced that incorporate interacting partner sequence at either end, which can be rendered suitable for deep sequencing (see Supplementary Methods).

  2. Validation of the assay.
    Figure 2: Validation of the assay.

    a, Graph showing an inverse relationship between interaction frequency and genomic distance (20kb or larger, excluding self-ligations and adjacent ligations) separating interacting restriction fragments (either HindIII or EcoRI) in each of four experimental but none of five control libraries. Note, the five lines representing the five control libraries are very close to each other. H-Mp, HindIII-MspI; H-Me, HindIII-MseI; E-Mp, EcoRI-MspI and E-Me, EcoRI-MseI library. b, The fraction of instances that each HindIII site along chromosome I (chr I) was engaged in an intra-chromosomal interaction was highly correlated between two independently derived experimental H-Mp (HindIII-MspI) libraries (designated A and B, left panel) but was not correlated between experimental and non-cross linked control H-Mp libraries (right panel). c, Two-dimensional heat maps demonstrating broad reproducibility of interaction patterns within chromosome I for two independently derived H-Mp libraries (H-Mp-A and the equivalent sequence depth of H-Mp-B, H-Mp-B1). The chromosomal positions of mappable (green hatches) and un-mappable (black hatches) HindIII fragments are indicated. The binary interaction matrix of all interactions with an FDR threshold of 1% has been smoothed with a Gaussian of width 3kb. d, High degree of correlation between absolute interaction frequencies as determined by our method (symbols) versus relative interaction frequencies as determined by conventional 3C using cross-linked (dark grey bars) and uncross-linked (light grey bars) libraries. Results for 10 potential long-range intra-chromosomal interactions are depicted, of which 6 passed (circles) and 4 did not pass (triangles) an FDR threshold of 1%. Error bars denote standard deviations over three experiments. Interaction sites are as follows. A, Chr III position 11811; B, chr III position 290056; C, chr III position 15939; D, chr III position 314440; E, chr I position 26147; F, chr I position 191604; G, chr I position 204567; H, chr VI position 12007; I, chr VI position 243206; J, chr VI position 249743; K, chr II position 238203; L, chr II position 502988; M, chr II position 512024; N, chr IV position 236977; O, chr IV position 447899; P, chr IV position 239805; Q, chr IV position 461284.

  3. Folding patterns of chromosomes.
    Figure 3: Folding patterns of chromosomes.

    Chromosomes III (a, b) and XII (c, d) are shown. The heat maps (a, c) and Circos diagrams (b, d) were generated using the intra-chromosomal interactions identified from the HindIII libraries at an FDR threshold of 1%. In the heat maps (a, c), the chromosomal positions of centromeres (dashed pink lines), telomeres (pink hatches), mappable (green hatches) and un-mappable (black hatches) HindIII fragments are indicated. Circos diagrams (b, d) depict each chromosome as a circle. Each arc connects two HindIII fragments and represents a distinct interaction. The shade of each arc, from very light grey to black, is proportional to the negative log of the P-value of the interaction. The chromosomal positions of centromeres (red rectangles), telomeres (red coloured areas), tRNA genes (blue outer hatches), mappable (green inner hatches) and un-mappable (black inner hatches) HindIII fragments are indicated. Black outer hatches and numbers mark genomic positions. Note that the two ends of chromosome XII (c, d) exhibit extensive local interactions, but very little interaction with each other. Separating the ends of chromosome XII are 100–200 rDNA repeats, of which only two copies are depicted here (from coordinates 450 to 470kb). Additional heat maps and Circos diagrams for all chromosomes are shown in Supplementary Fig. 8.

  4. Inter-chromosomal interactions.
    Figure 4: Inter-chromosomal interactions.

    a, Circos diagram showing interactions between chromosome I and the remaining chromosomes. All 16 yeast chromosomes are aligned circumferentially, and arcs depict distinct inter-chromosomal interactions. Bold red hatch marks correspond to centromeres. To aid visualization of centromere clustering, these representations were created using the overlap set of inter-chromosomal interactions identified from both HindIII and EcoRI libraries at an FDR threshold of 1%. Additional heat maps and Circos diagrams are provided in Supplementary Fig. 9. b, Circos diagram, generated using the inter-chromosomal interactions identified from the HindIII libraries at an FDR threshold of 1%, depicting the distinct interactions between a small and a large chromosome (I and XIV, respectively). Most of the interactions between these two chromosomes primarily involve the entirety of chromosome I, and a distinct region of corresponding size on chromosome XIV. c, Inter-chromosomal interactions between all pairs of the 32 yeast chromosomal arms (the 10kb region starting from the midpoint of the centromere in each arm is excluded). For each chromosome, the shorter arm is always placed before the longer arm. Note that the arms of small chromosomes tend to interact with one another. The colour scale corresponds to the natural log of the ratio of the observed versus expected number of interactions (see Supplementary Materials). d, Enrichment of interactions between centromeres, telomeres, early origins of replication, and chromosomal breakpoints. To measure enrichment of strong interactions with respect to a given class of genomic loci, we use receiver operating curve (ROC) analysis.

  5. Three-dimensional model of the yeast genome.
    Figure 5: Three-dimensional model of the yeast genome.

    Two views representing two different angles are provided. Chromosomes are coloured as in Fig. 4a (also indicated in the upper right). All chromosomes cluster via centromeres at one pole of the nucleus (the area within the dashed oval), while chromosome XII extends outward towards the nucleolus, which is occupied by rDNA repeats (indicated by the white arrow). After exiting the nucleolus, the remainder of chromosome XII interacts with the long arm of chromosome IV.

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Author information

  1. These authors contributed equally to this work.

    • Zhijun Duan &
    • Mirela Andronescu

Affiliations

  1. Institute for Stem Cell and Regenerative Medicine, University of Washington, Seattle, Washington 98195-8056, USA

    • Zhijun Duan,
    • Yoo Jung Kim &
    • C. Anthony Blau
  2. Department of Medicine, University of Washington Seattle, Washington 98195-8056, USA

    • Zhijun Duan,
    • Yoo Jung Kim,
    • Stanley Fields &
    • C. Anthony Blau
  3. Department of Genome Sciences, University of Washington, Seattle, Washington 98195-5065, USA

    • Mirela Andronescu,
    • Sean McIlwain,
    • Choli Lee,
    • Jay Shendure,
    • Stanley Fields,
    • C. Anthony Blau &
    • William S. Noble
  4. Graduate Program in Molecular and Cellular Biology, University of Washington, Seattle, Washington 98195-5065, USA

    • Kevin Schutz
  5. Howard Hughes Medical Institute

    • Stanley Fields

Contributions

Z.D. devised the strategy for characterizing genome architecture, Z.D., J.S, S.F, C.A.B. and W.S.N. designed experiments, Z.D., K.S., Y.J.K., and C.L. performed experiments, Z.D., M.A., S.M., J.S., S.F., C.A.B. and W.S.N. analysed experimental data, M.A., K.S., J.S. and W.S.N. commented on the manuscript drafts, Z.D., S.F., and C.A.B. wrote the paper.

Competing financial interests

The authors declare no competing financial interests.

Corresponding authors

Correspondence to:

Sequencing data have been deposited in the Sequence Read Archive under accession number SRP002120. An interactive website for yeast chromosomal interactions can be found at http://noble.gs.washington.edu/proj/yeast-architecture.

Author details

Supplementary information

PDF files

  1. Supplementary Information (11M)

    This file contains Supplementary Results, Methods, Data Analysis, References and Validation of Methods, Supplementary Tables 1- 4 and 14 -15 (for Supplementary Tables 5-13 see separate excel files) and Supplementary Figures 1-18 with legends. Minor errors in this file were corrected on 19 May 2010.

Excel files

  1. Supplementary Table 5 (6.6M)

    This file contains a list of intra-chromosomal interactions identified from HindIII libraries at the threshold of FDR 1%.

  2. Supplementary Table 6 (22.7M)

    This file contains a list of inter-chromosomal interactions identified from HindIII libraries at the threshold of FDR 1%.

  3. Supplementary Table 7 (3M)

    This file contains a list of intra-chromosomal interactions identified from EcoRI libraries at the threshold of FDR 1%.

  4. Supplementary Table 8 (6.7M)

    This file contains a list of inter-chromosomal interactions identified from EcoRI libraries at the threshold of FDR 1%.

  5. Supplementary Table 9 (27K)

    This file contains the statistical data with respect to intra- and inter-chromosomal interactions-HindIII.

  6. Supplementary Table 10 (54K)

    This file contains a list of intra-chromosomal interactions between the 20 and 30 kb regions of the ends of the chromosomes.

  7. Supplementary Table 11 (146K)

    This file contains a list of Inter-chromosomal telomere pairing.

  8. Supplementary Table 12 (27K)

    This file contains a list of primers used in this project.

  9. Supplementary Table 13 (392K)

    This file contains a list of mappable HindIII and EcoRI fragments in each chromosome.

Protein data bank files

  1. Supplementary Information (1.7M)

    This file contains a 3d model of the yeast genome. This file can be opened using Rasmol (http://rasmol.org/).

Comments

  1. Report this comment #10782

    Klaus Scherrer said:

    Dear Colleagues, felicitations for your most exciting article which, finally, may render the notion of the 3D nature of genomes acceptable to the many. It is also a perfect illustration of the Unified Matrix Hypothesis (UMH), which tried to draw attention to the 3D organization of DNA based on data available 20 years ago (Scherrer, Bioscience Reports1989, 9, 157-188). Sparked off by an attempt to cope with the C-value paradox (and the nonsense of ?junk DNA? !), essential is the phenomenon of ectopic pairing of drosophila polytene chromosomes where a 3D genomic network is visible in the microscope, including the position of the nucleolus in a manner compatible with your actual model. An essential point is that, hence, mere DNA length, independent of sequence, represents another type of genetic information, in addition to the genetic code and the sequential arrangement of CRMs in DNA and RNA recognized by regulatory factors and/or si/miRNA (c.f. Gene and Genon Concept; doi:10.1038/msb4100123). This type of genomic information also may give a rationale to the existence and nature of repetitive DNA.

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