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Nature 461, 1069-1070 (22 October 2009) | doi:10.1038/4611069a; Published online 21 October 2009
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Professor of Experimental Virology (W3)
- University Hospital Jena, Institute of Virology and Antivirale Therapy
- Jena, Germany
Tenure-track Faculty Positions
- University of Michigan
- Ann Arbor, Michigan, USA
Microscopy: Light from the dark
Stefan W. Hell1 & Eva Rittweger1
Abstract
Fluorescence microscopy is the most popular way to image biomolecules, but it leaves many of them in the dark. Non-fluorescent, light-absorbing molecules can now be viewed by a method that turns them into mini-lasers.
What happens when a molecule absorbs a photon from a beam of light? It moves from the ground state to an excited, higher-energy state and then quickly relaxes, giving off the absorbed energy as heat.
- Stefan W. Hell and Eva Rittweger are in the Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, 37070 Göttingen, Germany. Stefan W. Hell is also at the German Cancer Research Center (DKFZ), Heidelberg.
Email: hell@nanoscopy.de
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