Supplementary information
From the following article:
Coordination of Rho GTPase activities during cell protrusion
Matthias Machacek, Louis Hodgson, Christopher Welch, Hunter Elliott, Olivier Pertz, Perihan Nalbant, Amy Abell, Gary L. Johnson, Klaus M. Hahn & Gaudenz Danuser
Nature 461, 99-103(3 September 2009)
doi:10.1038/nature08242
Supplementary Information
This file contains Supplementary Methods, Supplementary Data, Supplementary Figures S1-S10 with Legends and Supplementary References.
Supplementary Movie 1
This Movie shows the Rac1 activation measured using the FRET/CyPet ratio to monitor interaction between Rac1 and the p21-binding domain from PAK1 (see Figs. 1a, S1a). Left panel: corresponding time points imaged by DIC. Frame interval: 10 s. Replay: 10 frames/s. Duration of original sequence: 20 min. Magnification 40x, 2x2 binning. Scale bar: 20 
m. Colour-bar defines the dynamic range of the corrected FRET/CyPet ratio.
Supplementary Movie 2
This Movie shows the zoom of the protrusive region of Movie 1. This sector of the cell edge is analyzed in Fig. 2.
Supplementary Movie 3
This Movie shows the Cdc42 activation measured using the meroCBD biosensor (see Figs. 1b, S1b). Left panel: corresponding time points imaged by DIC. Frame interval: 10 s. Duration of original sequence: 13 min. Replay: 10 frames/s. Magnification 40x, 2x2 binning. Scale bar: 20 m. Colour-bar defines the dynamic range of the merocyanine dye/EGFP ratio.
Supplementary Movie 4
This Movie shows the zoom of the protrusive region of Movie 3. This sector of the cell edge is analyzed in Fig. 2.
Supplementary Movie 5
This Movie shows the RhoA activation measured using the single chain, intramolecular FRET biosensor (see Figs. 1c, S1c). Left panel: corresponding time points imaged by DIC. Frame interval: 10 s. Replay: 10 frames/s. Duration of original sequence: 10 min. Magnification 40x, 2x2 binning. Scale bar: 20 m. Colour-bar defines the dynamic range of the FRET/CFP ratio.
Supplementary Movie 6
This Movie shows the zoom of the protrusive region of Movie 5. This sector of the cell edge is analyzed in Fig. 2.
Supplementary Movie 7
This Movie shows sampling windows of 1.8 m width and 0.9 m depth placed at 0 m and 2.5 m from the edge follow the cell morphology. Windows are overlaid on the time lapse sequence shown in Movie 4. Frame interval: 10s. Replay: 10 frames/s.
Supplementary Movie 8
This Movie shows the RhoA activation measured using the intermolecular dual chain FRET biosensor (see Figs. S1d, S10). Frame interval: 10 s. Replay: 10 frames/s. Magnification 40x, 2x2 binning. Scale bar: 20 m. Colour-bar defines the dynamic range of the corrected FRET/CyPet ratio.
Supplementary Movie 9
This Movie shows the zoomed view of protrusion and retraction activity of a randomly migrating MEF imaged simultaneously using Cdc42 and RhoA biosensors (see Fig. S7 for snapshots of the full view time sequence). Frame interval: 10s. Replay: 10 frames/s. Magnification 40x, 2x2 binning. Scale-bar: 15 m. Colour-bar defines the dynamic range of the Cdc42 and RhoA signals.
DOWNLOAD BROWSER PLUGINS AND OTHER APPLICATIONS
Movie files
PDF douments
Text documents
PostScript documents
Flash movies
Audio files
- Apple iTunes (PC or Mac)
- QuickTime Player (PC or Mac)
- Realplayer (PC or Mac)
- Windows Media player (PC only)
