The technique of second harmonic generation microscopy is used to obtain pictures of living systems, but the dyes required provide only modest imaging contrast per molecule. The latest dyes give a much better picture.
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Therien, M. How to improve your image. Nature 458, 716–717 (2009). https://doi.org/10.1038/458716b
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DOI: https://doi.org/10.1038/458716b
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