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Nature 458, 102-105 (5 March 2009) | doi:10.1038/nature07814;
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Protein structure determination in living cells by in-cell NMR spectroscopy
Investigating proteins |[lsquo]|at work|[rsquo]| in a living environment at atomic resolution is a major goal of molecular biology, which has not been achieved even though methods for the three-dimensional (3D) structure determination of purified proteins in single crystals or in solution are widely used. Recent developments in NMR hardware and methodology have enabled the measurement of high-resolution heteronuclear multi-dimensional NMR spectra of macromolecules in living cells (in-cell NMR).
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