Article

Nature 457, 551-556 (29 January 2009) | doi:10.1038/nature07723; Received 20 August 2008; Accepted 9 December 2008

The Sorghum bicolor genome and the diversification of grasses

Andrew H. Paterson1, John E. Bowers1, Rémy Bruggmann2, Inna Dubchak3, Jane Grimwood4, Heidrun Gundlach5, Georg Haberer5, Uffe Hellsten3, Therese Mitros6, Alexander Poliakov3, Jeremy Schmutz4, Manuel Spannagl5, Haibao Tang1, Xiyin Wang1,7, Thomas Wicker8, Arvind K. Bharti2, Jarrod Chapman3, F. Alex Feltus1,9, Udo Gowik10, Igor V. Grigoriev3, Eric Lyons11, Christopher A. Maher12, Mihaela Martis5, Apurva Narechania12, Robert P. Otillar3, Bryan W. Penning13, Asaf A. Salamov3, Yu Wang5, Lifang Zhang12, Nicholas C. Carpita14, Michael Freeling11, Alan R. Gingle1, C. Thomas Hash15, Beat Keller8, Patricia Klein16, Stephen Kresovich17, Maureen C. McCann13, Ray Ming18, Daniel G. Peterson1,19, Mehboob-ur-Rahman1,20, Doreen Ware12,21, Peter Westhoff10, Klaus F. X. Mayer5, Joachim Messing2 & Daniel S. Rokhsar3,4

  1. Plant Genome Mapping Laboratory, University of Georgia, Athens, Georgia 30602, USA
  2. Waksman Institute for Microbiology, Rutgers University, Piscataway, New Jersey 08854, USA
  3. DOE Joint Genome Institute, Walnut Creek, California 94598, USA
  4. Stanford Human Genome Center, Stanford University, Palo Alto, California 94304, USA
  5. MIPS/IBIS, Helmholtz Zentrum München, Inglostaedter Landstrasse 1, 85764 Neuherberg, Germany
  6. Center for Integrative Genomics, University of California, Berkeley, California 94720, USA
  7. College of Sciences, Hebei Polytechnic University, Tangshan, Hebei 063000, China
  8. Institute of Plant Biology, University of Zurich, Zollikerstrasse 107, 8008 Zurich, Switzerland
  9. Department of Genetics and Biochemistry, Clemson University, Clemson, South Carolina 29631, USA
  10. Institut fur Entwicklungs und Molekularbiologie der Pflanzen, Heinrich-Heine-Universitat, Universitatsstrasse 1, D-40225 Dusseldorf, Germany
  11. Department of Plant and Microbial Biology, University of California, Berkeley, California 94720, USA
  12. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, USA
  13. Department of Biological Sciences,
  14. Department of Botany and Plant Pathology, Purdue University, West Lafayette, Indiana 47907, USA
  15. International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Patancheru 502 324, India
  16. Department of Horticulture and Institute for Plant Genomics and Biotechnology, Texas A&M University, College Station, Texas 77843, USA
  17. Institute for Genomic Diversity, Cornell University, Ithaca, New York 14853, USA
  18. Department of Plant Biology, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801, USA
  19. Mississippi Genome Exploration Laboratory, Mississippi State University, Starkville, Mississippi 39762, USA
  20. National Institute for Biotechnology & Genetic Engineering (NIBGE), Faisalabad, Pakistan
  21. USDA NAA Robert Holley Center for Agriculture and Health, Ithaca, New York 14853, USA

Correspondence to: Andrew H. Paterson1 Correspondence and requests for materials should be addressed to A.H.P. (Email: paterson@uga.edu).

This article is distributed under the terms of the Creative Commons Attribution-Non-Commercial-Share Alike licence (http://creativecommons.org/licenses/by-nc-sa/3.0/), which permits distribution, and reproduction in any medium, provided the original author and source are credited. This license does not permit commercial exploitation, and derivative works must be licensed under the same or similar licence.

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Sorghum, an African grass related to sugar cane and maize, is grown for food, feed, fibre and fuel. We present an initial analysis of the approx730-megabase Sorghum bicolor (L.) Moench genome, placing approx98% of genes in their chromosomal context using whole-genome shotgun sequence validated by genetic, physical and syntenic information. Genetic recombination is largely confined to about one-third of the sorghum genome with gene order and density similar to those of rice. Retrotransposon accumulation in recombinationally recalcitrant heterochromatin explains the approx75% larger genome size of sorghum compared with rice. Although gene and repetitive DNA distributions have been preserved since palaeopolyploidization approx70 million years ago, most duplicated gene sets lost one member before the sorghum–rice divergence. Concerted evolution makes one duplicated chromosomal segment appear to be only a few million years old. About 24% of genes are grass-specific and 7% are sorghum-specific. Recent gene and microRNA duplications may contribute to sorghum's drought tolerance.

The Saccharinae plants include some of the most efficient biomass accumulators, providing food and fuel from starch (sorghum) and sugar (sorghum and Saccharum, sugar cane), and have potential for use as cellulosic biofuel crops (sorghum, sugar cane, Miscanthus). Of singular importance to Saccharinae productivity is C4 photosynthesis, comprising biochemical and morphological specializations that increase net carbon assimilation at high temperatures1. Despite their common photosynthetic strategy, the Saccharinae show much morphological and genomic variation (Supplementary Fig. 1).

Its small genome (approx730 Mb) makes sorghum an attractive model for functional genomics of Saccharinae and other C4 grasses. Rice, the first fully sequenced cereal genome, is more representative of C3 photosynthetic grasses. Drought tolerance makes sorghum especially important in dry regions such as northeast Africa (its centre of diversity) and the southern plains of the United States. Genetic variation in the partitioning of carbon into sugar stores versus cell wall mass, and in perenniality and associated features such as tillering and stalk reserve retention2, make sorghum an attractive system for the study of traits important in perennial cellulosic biomass crops. Its high level of inbreeding makes it an attractive association genetics system3. Transgenic approaches to sorghum improvement are constrained by high gene flow to weedy relatives4, making knowledge of its intrinsic genetic potential all the more important.

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Reconstructing a repeat-rich genome from shotgun sequences

Preferred approaches to sequencing entire genomes are currently to apply shotgun sequencing5 either to a minimum 'tiling path' of genomic clones, or to genomic DNA directly. The latter approach, whole-genome shotgun (WGS) sequencing, is widely used for mammalian genomes, being fast, relatively economical and reducing cloning bias. However, its applicability has been questioned for repetitive DNA-rich plant genomes6.

Despite a repeat content of approx61%, a high-quality genome sequence was assembled from homozygous sorghum genotype BTx623 by using WGS and incorporating the following: (1) approx8.5 genome equivalents of paired-end reads7 from genomic libraries spanning a approx100-fold range of insert sizes (Supplementary Table 1), resolving many repetitive regions; and (2) high-quality read length averaging 723 bp, facilitating assembly. Comparison with 27 finished bacterial artificial chromosomes (BACs) showed the WGS assembly to be >98.46% complete and accurate to <1 error per 10 kb (Supplementary Note 2.5).

Comparison with a high-density genetic map8, a 'finger-print contig' (FPC)-based physical map9, and the rice sequence6 improved the sorghum WGS assembly (Supplementary Notes 1 and 2). Among the 201 largest scaffolds (spanning 678.9 Mb, 97.3% of the assembly), 28 showed discrepancies with two or more of these lines of evidence (Supplementary Note 2.6), often near repetitive elements. After breaking the assembly at the points of discrepancy, the resulting 229 scaffolds have an N50 (number of scaffolds that collectively cover at least 50% of the assembly) of 35 and L50 (length of the shortest scaffold among those that collectively cover 50% of the assembly) of 7.0 Mb. A total of 38 (2%) of 1,869 FPC contigs9 were deemed erroneous, containing >5 BAC ends that fell into different sequence scaffolds.

A total of 127 scaffolds containing 625.7 Mb (89.7%) of DNA and 1,476 FPC contigs could be assigned to chromosomal locations and oriented. Fifteen out of twenty chromosome ends terminated in telomeric repeats. The other 102 scaffolds were generally smaller (53.2 Mb, 7.6%), with 85 (83%) containing far greater-than-average abundance of the Cen38 (ref. 10) centromeric repeat, and with only 374 predicted genes. These 102 scaffolds merged only 193 FPC contigs, presumably due to the greater abundance of repeats that are recalcitrant to clone-based physical mapping9 and may be omitted in BAC-by-BAC approaches11.

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Genome size evolution and its causes

The approx75% larger quantity of DNA in the genome of sorghum compared with rice is mostly heterochromatin. Alignment to genetic8 and cytological maps12 suggests that sorghum and rice have similar quantities of euchromatin (252 and 309 Mb, respectively; Supplementary Table 7), accounting for 97–98% of recombination (1,025.2 cM and 1,496.5 cM, respectively) and 75.4–94.2% of genes in the respective cereals, with largely collinear gene order9. In contrast, sorghum heterochromatin occupies at least 460 Mb (62%), far more than in rice (63 Mb, 15%). The approx3times genome expansion in maize since its divergence from sorghum13 has been more dispersed—recombinogenic DNA has grown 4.5times to approx1,382 Mb, much more than can be explained by genome duplication14.

The net size expansion of the sorghum genome relative to rice largely involved long terminal repeat (LTR) retrotransposons. The sorghum genome contains 55% retrotransposons, intermediate between the larger maize genome (79%) and smaller rice genome (26%). Sorghum more closely resembles rice in having a higher ratio of gypsy-like to copia-like elements (3.7 to 1 and 4.9 to 1) than maize (1.6 to 1: Supplementary Table 10).

Although recent retroelement activity is widely distributed across the sorghum genome, turnover is rapid (as in other cereals15) with pericentromeric elements persisting longer. Young LTR retrotransposon insertions (<0.01 million years (Myr) ago) appear randomly distributed along chromosomes, suggesting that they are preferentially eliminated from gene-rich regions9 but accumulate in gene-poor regions (Fig. 1; see also Supplementary Note 3.1). Insertion times suggest a major wave of retrotransposition <1 Myr ago, after a smaller wave 1–2 Myr ago (Supplementary Fig. 2).

Figure 1: Genomic landscape of sorghum chromosomes 3 and 9.
Figure 1 : Genomic landscape of sorghum chromosomes 3 and 9. Unfortunately we are unable to provide accessible alternative text for this. If you require assistance to access this image, or to obtain a text description, please contact npg@nature.com

Area charts quantify retrotransposons (55%), genes (6% exons, 8% introns), DNA transposons (7%) and centromeric repeats (2%). Lines between chromosomes 3 and 9 connect collinear duplicated genes. Heat-map tracks detail the distribution of selected elements. Figures for all sorghum chromosomes are in Supplementary Note 3. Cen38, sorghum-specific centromeric repeat10; RTs, retrotransposons (class I); LTR-RTs, long terminal repeat retrotransposons; DNA-TEs, DNA transposons (class II).

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CACTA-like elements, the predominant sorghum DNA transposons (4.7% of the genome), seem to relocate genes and gene fragments, as do rice 'Pack-MULEs'16 and maize helitrons17. Many sorghum CACTA elements are non-autonomous deletion derivatives in which transposon genes have been replaced with non-transposon DNA including exons from one or more cellular genes as exemplified for family G118 (Fig. 2). Among 13,775 CACTA elements identified (Supplementary Note 3.4), 200 encode no transposon proteins but contain at least one cellular gene fragment.

Figure 2: CACTA element deletion derivatives that carry gene fragments.
Figure 2 : CACTA element deletion derivatives that carry gene fragments. Unfortunately we are unable to provide accessible alternative text for this. If you require assistance to access this image, or to obtain a text description, please contact npg@nature.com

CACTA family G118 has only one complete and presumably autonomous 'mother' element. Among 18 deletion derivatives, only the terminal 500–2,500 bp are conserved, with 8 carrying gene fragments internally. One relatively homogeneous subgroup (106, 111 and 112) presumably arose recently, whereas other derivatives are unique. The locations of the hits to known rice proteins are indicated as coloured boxes. The descriptions of the foreign gene fragments are indicated underneath the boxes. HP, hypothetical protein.

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In total, DNA transposons constitute 7.5% of the sorghum genome, intermediate between maize (2.7%) and rice (13.7%; Supplementary Table 10). Miniature inverted-repeat transposable elements, 1.7% of the genome, are associated with genes (Fig. 1; see also Supplementary Note 3) as in other cereals6. Helitrons, approx0.8% of the genome, nearly all lack helicase in sorghum as in maize17, but carry fewer gene fragments in sorghum than maize (Supplementary Note 3.5). Organellar DNA insertion has contributed only 0.085% to the sorghum nuclear genome, far less than the 0.53% of rice (Supplementary Note 2.7).

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The gene complement of sorghum

Among 34,496 sorghum gene models, we found approx27,640 bona fide protein-coding genes by combining homology-based and ab initio gene prediction methods with expressed sequences from sorghum, maize and sugar cane (Supplementary Note 4). Evidence for alternate splicing is found in 1,491 loci.

Another 5,197 gene models are usually shorter than the bona fide genes (often <150 amino acids); have few exons (often one) and no expressed sequence tag (EST) support (compared with 85% for bona fide genes); are more diverged from rice genes; and are often found in large families with 'hypothetical', 'uncharacterized' and/or retroelement-associated annotations, despite repeat masking (Supplementary Note 4). A high concentration in pericentromeric regions where bona fide genes are scarce (Fig. 1) suggests that many of these low confidence gene models are retroelement-derived. We also identified 727 processed pseudogenes and 932 models containing domains known only from transposons.

The exon size distributions of orthologous sorghum and rice genes agree closely, and intron position and phase show >98% concordance (Supplementary Note 5). Intron size has been conserved between sorghum and rice, although it has increased in maize owing to transpositions18.

Most paralogues in sorghum are proximally duplicated, including 5,303 genes in 1,947 families of greater than or equal to2 genes (Supplementary Note 4.3). The longest tandem gene array is 15 cytochrome P450 genes. Other sorghum-specific tandem gene expansions include haloacid dehalogenase-like hydrolases (PF00702), FNIP repeats (PF05725), and male sterility proteins (PF03015).

We confirmed the genomic locations of 67 known sorghum microRNAs (miRNAs) and identified 82 additional miRNAs (Supplementary Note 4.4). Five clusters located within 500 bp of each other represent putative polycistronic miRNAs, similar to those in Arabidopsis and Oryza. Natural antisense miRNA precursors (nat-miRNAs) of family miR444 (ref. 19) have been identified in three copies.

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Comparative gene inventories of angiosperms

The number and sizes of sorghum gene families are similar to those of Arabidopsis, rice and poplar (Fig. 3 and Supplementary Note 4.6). A total of 9,503 (58%) sorghum gene families were shared among all four species and 15,225 (93%) with at least one other species. Nearly 94% (25,875) of high-confidence sorghum genes have orthologues in rice, Arabidopsis and/or poplar, and together these gene complements define 11,502 ancestral angiosperm gene families represented in at least one contemporary grass and rosid genome. However, 3,983 (24%) gene families have members only in the grasses sorghum and rice; 1,153 (7%) appear to be unique to sorghum.

Figure 3: Orthologous gene families between sorghum, Arabidopsis, rice and poplar.
Figure 3 : Orthologous gene families between sorghum, Arabidopsis, rice and poplar. Unfortunately we are unable to provide accessible alternative text for this. If you require assistance to access this image, or to obtain a text description, please contact npg@nature.com

The numbers of gene families (clusters) and the total numbers of clustered genes are indicated for each species and species intersection.

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Pfam domains that are over-represented, under-represented or even absent in sorghum relative to rice, poplar and Arabidopsis, may reflect biological peculiarities specific to the Sorghum lineage (Supplementary Table 20). Domains over-represented in sorghum are usually present in the other organisms, a notable exception being the alpha-kafirin domain that accounts for most seed storage protein and corresponds to maize zeins20 but which is absent from rice.

Nucleotide-binding-site–leucine-rich-repeat (NBS-LRR) containing proteins associated with the plant immune system are only about half as frequent in sorghum as in rice. A search with 12 NBS domains from published rice, maize, wheat and Arabidopsis gene sequences revealed 211 NBS-LRR coding genes in sorghum, 410 in rice and 149 in Arabidopsis21. Sorghum NBS-LRR genes mostly encode the CC type of N-terminal domains. Only two sorghum genes (Sb02g005860 and Sb02g036630) contain the TIR domain, and neither contains an NBS domain. NBS-LRR genes are most abundant on sorghum chromosome 5 (62), and its rice homologue (chromosome 11, 106). Enrichment of NBS-LRR genes in these corresponding genomic regions suggests conservation of R gene location, in contrast to a proposal that R gene movement may be advantageous22.

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Evolution of distinctive pathways and processes

The evolution of C4 photosynthesis in the Sorghum lineage involved redirection of C3 progenitor genes as well as recruitment and functional divergence of both ancient and recent gene duplicates. The sole sorghum C4 pyruvate orthophosphate dikinase (ppdk) and the phosphoenolpyruvate carboxylase kinase (ppck) gene and its two isoforms (produced by the whole genome duplication) have only single orthologues in rice. Additional duplicates formed in maize after the sorghum–maize split (Zmppck2 and Zmppck3). The C4 NADP-dependent malic enzyme (me) gene has an adjacent isoform but each corresponds to a different maize homologue, suggesting tandem duplication before the sorghum–maize split. The C4 malate dehydrogenase (mdh) gene and its isoform are also adjacent, but share 97% amino acid similarity and correspond to the single known maize Mdh gene, suggesting tandem duplication in sorghum after its split with maize. The rice Me and Mdh genes are single copy, suggesting duplication and recruitment to the C4 pathway after the Panicoideae–Oryzoideae divergence (Supplementary Note 9).

The sorghum sequence reinforces inferences previously based only on rice, about how different grass and dicotyledon gene inventories relate to their respective types of cell walls23, 24. In grasses, cellulose microfibrils coated with mixed-linkage (1right arrow3),(1right arrow4)-beta-d-glucans are interlaced with glucuronoarabinoxylans and an extensive complex of phenylpropanoids25. The sorghum sequence largely corroborates differences between dicotyledons and rice in the distribution of cell wall biogenesis genes (Supplementary Note 10). For example, the CesA/Csl superfamily and callose synthases have either diverged to form new subgroups or functionally non-essential subgroups were selectively lost, such as CslB and CslG lost from the grasses, and CslF and CslH lost from species with dicotyledon-like cell walls26. The previously rice-unique CslF and CslH genes are present in sorghum. Arabidopsis contains a single group F GT31 gene, whereas sorghum and rice contain six and ten, respectively.

The characteristic adaptation of sorghum to drought may be partly related to expansion of one miRNA and several gene families. Rice miRNA 169g, upregulated during drought stress27, has five sorghum homologues (sbi-MIR169c, sbi-MIR169d, sbi-MIR169.p2, sbi-MIR169.p6 and sbi-MIR169.p7). The computationally predicted target of the sbi-MIR169 subfamily comprises members of the plant nuclear factor Y (NF-Y) B transcription factor family, linked to improved performance under drought by Arabidopsis and maize28. Cytochrome P450 domain-containing genes, often involved in scavenging toxins such as those accumulated in response to stress, are abundant in sorghum with 326 versus 228 in rice. Expansins, enzymes that break hydrogen bonds and are responsible for a variety of growth responses that could be linked to the durability of sorghum, occur in 82 copies in sorghum versus 58 in rice and 40 each in Arabidopsis and poplar.

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Duplication and diversification of cereal genomes

Whole-genome duplication in a common ancestor of cereals is reflected in sorghum and rice gene 'quartets' (Fig. 4). A total of 19,929 (57.8%) sorghum gene models were in blocks collinear with rice (Supplementary Note 6). After the shared whole-genome duplication, only one copy was retained for 13,667 (68.6%) collinear genes with 13,526 (99%) being orthologous in rice–sorghum, indicating that most gene losses predate taxon divergence. Both sorghum and rice retained both copies of 4,912 (14.2%) genes, whereas sorghum lost one copy of 1,070 (3.1%) and rice lost one copy of 634 (1.8%). These patterns are likely to be predictive of other grass genomes, as the major grass lineages diverged from a common ancestor at about the same time29 (see also Supplementary Note 7).

Figure 4: Alignment of sorghum, rice and maize.
Figure 4 : Alignment of sorghum, rice and maize. Unfortunately we are unable to provide accessible alternative text for this. If you require assistance to access this image, or to obtain a text description, please contact npg@nature.com

Dot plots show intergenomic (gold) and intragenomic (black) alignments. One sorghum–rice quartet showing both orthologous and paralogous (duplicated) regions is magnified. Infrequent gene loss (red; see legend) after sorghum–rice divergence causes 'special cases' in which there are paralogues but no orthologues. Each sorghum region corresponds to two duplicated maize regions39, with maize gene loss suggested where sorghum loci only match one of the two. Because maize BACs are mostly unfinished, sorghum loci are aligned to the centres. Note the different scale necessary for maize physical distance. Larger dot plots are in Supplementary Note 6.

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Although most post-duplication gene loss happened in a common cereal ancestor, some lineage-specific patterns occur. A total of 2 and 10 protein functional (Pfam) domains showed enrichment for duplicates and singletons (respectively) in sorghum but not rice (Supplementary Note 6.1). Because the sorghum–rice divergence is thought to have happened 20 Myr or more after genome duplication29, this suggests that even long-term gene loss differentially affects gene functional groups.

One genomic region has been subject to a high level of concerted evolution. It was previously suggested that rice chromosomes 11 and 12 share a approx5–7-Myr-old segmental duplication30, 31, 32. We found a duplicated segment in the corresponding regions of sorghum chromosomes 5 and 8 (Fig. 5). Sorghum–sorghum and rice–rice paralogues from this region show rates of synonymous DNA substitution (Ks) of 0.44 and 0.22, respectively, consistent with only 34 and 17 Myr of divergence. However, the Ks value of sorghum–rice orthologues is 0.63, similar to the respective genome-wide averages (0.81, 0.87). We hypothesize that the apparent segmental duplication actually resulted from the pan-cereal whole-genome duplication and became differentiated from the remainder of the chromosome(s) owing to concerted evolution acting independently in sorghum, rice and perhaps other cereals. Gene conversion and illegitimate recombination are more frequent in the rice 11–12 region than elsewhere in the genome33. Physical and genetic maps suggest shared terminal segments of the corresponding chromosomes in wheat (4, 5)34, foxtail millet (VII, VIII) and pearl millet (linkage groups 1, 4)35.

Figure 5: Independent illegitimate recombination in corresponding regions of sorghum and rice.
Figure 5 : Independent illegitimate recombination in corresponding regions of sorghum and rice. Unfortunately we are unable to provide accessible alternative text for this. If you require assistance to access this image, or to obtain a text description, please contact npg@nature.com

Four homologous rice and sorghum chromosomes (11 and 12 in rice; 5 and 8 in sorghum) are shown, with gene densities plotted. 'L' and 'S' show long and short arms, respectively. Lines show Ks between homologous gene pairs, and colours are used to show different dates of conversion events.

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Synthesis and implications

Comparison of the sorghum, rice and other genomes clarifies the grass gene set. Pairs of orthologous sorghum and rice genes combined with recent paralogous duplications define 19,542 conserved grass gene families, each representing one gene in the sorghum–rice common ancestor. Our sorghum gene count is similar to that in a manually curated rice annotation (RAP2)36, but this similarity masks some differences. About 2,054 syntenic orthologues shared by our sorghum annotation and the TIGR5 (ref. 37) rice annotation are absent from RAP2. Conversely, approx12,000 TIGR5 annotations may be transposable elements or pseudogenes, comprising large families of hypothetical genes in both sorghum and rice RAP2, often with short exons, few introns and limited EST support. Phylogenetically incongruent cases of apparent gene loss (for example, genes shared by Arabidopsis and sorghum but not rice: Fig. 3) may also suggest sequence gaps or misannotations.

Grass genome architecture may reflect euchromatin-specific effects of recombination and selection, superimposed on non-adaptive processes of mutation and genetic drift that apply to all genomic regions38. Patterns of gene and repetitive DNA organization remain correlated in homologous chromosomes duplicated 70 Myr ago (Fig. 1), despite extensive turnover of specific repetitive elements. Synteny is highest and retroelement abundance lowest in distal chromosomal regions. More rapid retroelement removal from gene-rich euchromatin that frequently recombines than from heterochromatin that rarely recombines supports the hypothesis that recombination may preserve gene structure, order and/or spacing by exposing new insertions to selection9. Less euchromatin–heterochromatin polarization in maize, where retrotransposon persistence in euchromatin seems more frequent, may reflect variation in grass genome architecture or perhaps a lingering consequence of more recent genome duplication39.

Identification of conserved DNA sequences may help us to understand essential genes and binding sites that define grasses. Progress in sequencing Brachypodium distachyon40 sets the stage for panicoid–oryzoid–pooid phylogenetic triangulation of genomic changes, as well as association of some such changes with phenotypes ranging from molecular (gene expression patterns) to morphological. The divergence between sorghum, rice and Brachypodium is sufficient to randomize nonfunctional sequence, facilitating conserved noncoding sequence (CNS) discovery41, 42 (Supplementary Fig. 9). More distant comparisons to the dicotyledon Arabidopsis show exon conservation but no CNS (Supplementary Fig. 10). Chloridoid and arundinoid genome sequences are needed to sample the remaining grass lineages, and an outgroup such as Ananas (pineapple) or Musa (banana) would further aid in identifying genes and sequences that define grasses.

The fact that the sorghum genome has not re-duplicated in approx70 Myr29 makes it a valuable outgroup for deducing fates of gene pairs and CNS in grasses that have reduplicated. Single sorghum regions correspond to two regions resulting from maize-specific genome doubling39—gene fractionation is evident (Fig. 4), and subfunctionalization is probable (Supplementary Fig. 10). Sorghum may prove especially valuable for unravelling genome evolution in the more closely related SaccharumMiscanthus clade: two genome duplications since its divergence from sorghum 8–9 Myr ago43 complicate sugar cane genetics44 yet Saccharum BACs show substantially conserved gene order with sorghum (Supplementary Note 11).

Conservation of grass gene structure and order facilitates development of DNA markers to support crop improvement. We identified approx71,000 simple-sequence repeats (SSRs) in sorghum (Supplementary List 1); among a sampling of 212, only 9 (4.2%) map to paralogues of their source locus. Conserved-intron scanning primers (Supplementary List 2) for 6,760 genes provide DNA markers useful across many monocotyledons, particularly valuable for 'orphan cereals'45.

As the first sequenced plant genome of African origin, sorghum adds new dimensions to ethnobotanical research. Of particular interest will be the identification of alleles selected during the earliest stages of sorghum cultivation, which are valuable towards testing the hypothesis that convergent mutations in corresponding genes contributed to independent domestications of divergent cereals46. Invigorated sorghum improvement would benefit regions such as the African 'Sahel' where drought tolerance makes sorghum a staple for human populations that are increasing by 2.8% per year. Sorghum yield improvement has lagged behind that of other grains, in Africa only gaining a total of 37% (western) to 38% (eastern) from 1961–63 to 2005–07 (Supplementary Note 12).

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Methods Summary

Genome sequencing

Approximately 8.5-fold redundant paired-end shotgun sequencing was performed using standard Sanger methodologies from small (approx2–3 kb) and medium (5–8 kb) insert plasmid libraries, one fosmid library (approx35 kb inserts), and two BAC libraries (insert size 90 and 108 kb). (Supplementary Note 1.)

Integration of shotgun assembly with genetic and physical maps

The largest 201 scaffolds, all exceeding 39 kb, excluding 'N's, and collectively representing 678,902,941 bp (97.3%) of nucleotides, were checked for possible chimaeras suggested by the sorghum genetic map, sorghum physical map, abrupt changes in gene or repeat density, rice gene order, and coverage by BAC or fosmid clones (Supplementary Note 2).

Repeat analysis

De novo searches for LTR retrotransposons used LTR_STRUC. De novo detection of CACTA-DNA transposons and MITEs used custom programs (Supplementary Note 3). Known repeats were identified by RepeatMasker (Open-3-1-8) (http://www.repeatmasker.org) with mips-REdat_6.2_Poaceae, a compilation of grass repeats including sorghum-specific LTR retrotransposons (http://mips.gsf.de/proj/plant/webapp/recat/). The insertion age of full-length LTR-retrotransposons was determined from the evolutionary distance between 5' and 3' soloLTR derived from a ClustalW alignment of the two soloLTRs.

Protein-coding gene annotation

Putative protein-coding loci were identified based on BLAST alignments of rice and Arabidopsis peptides and sorghum and maize ESTs. GenomeScan47 was applied using maize-specific parameters. Predicted coding structures were merged with EST data from maize and sorghum using PASA48.

Intergenomic and intragenomic alignments

Dot plots used ColinearScan49 and multi-alignments used MCScan50, applied to RAP236 (mapped representative models, 29,389 loci) and the sbi1.4 annotation set (34,496 loci). Pairwise BLASTP (E < 1times10-5, top five hits), both within each genome and between the two genomes, was used to retrieve potential anchors. Zea BAC sequences and FPC contig coordinates were downloaded (http://www.maizesequence.org, release 7 January 2008). Zea BACs were searched for potential orthologues of Sorghum coding sequences using translated BLAT with a minimum score of 100.

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References

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Supplementary Information

Supplementary information accompanies this paper.

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Acknowledgements

We thank the US Department of Energy Joint Genome Institute Community Sequencing Program, J. Bristow, S. Lucas and the JGI production sequencing team for sequencing sorghum; and L. Lin for contributions to Fig. 1. We appreciate funding from the US National Science Foundation (NSF DBI-9872649, 0115903; MCB-0450260), International Consortium for Sugarcane Biotechnology, National Sorghum Producers, and a John Simon Guggenheim Foundation fellowship to A.H.P.; US Department of Energy (DE-FG05-95ER20194) to J.M.; German Federal Ministry of Education GABI initiative to MIPS (0313117 and 0314000C); NSF DBI-0321467 to A.N.; and US Department of Agriculture-Agricultural Research Service to C.A.M., L.Z. and D.W.

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