Supplementary information

From the following article:

The dynein regulatory complex is required for ciliary motility and otolith biogenesis in the inner ear

Jessica R. Colantonio, Julien Vermot, David Wu, Adam D. Langenbacher, Scott Fraser, Jau-Nian Chen & Kent L. Hill

Nature 457, 205-209(8 January 2009)

doi:10.1038/nature07520

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Supplementary Figures and legends

This file contains Supplementary Figures S1-S6 with legends, and legends for Supplementary Movies 1-11b.

Supplementary movie 1

Supplemental movie 1. Three dimensional display of a control inner ear at stage 27 hpf after immunofluorescence labelling of cilia with acetylated tubulin antibody.

Supplementary movie 2

Supplemental movie 2. Three dimensional display of a gas11 morphant inner ear at stage 27 hpf after acetylated tubulin immunofluorescence labelling.

Supplementary movie 3

Supplemental movie 3. Video shows tether cilia motility in control embryo.

Supplementary movie 4

Supplemental movie 4. Video shows tether cilia motility in control embryo.

Supplementary movie 5

Supplemental movie 5. Video shows tether cilia motility in control embryo.

Supplementary movie 6

Supplemental movie 6. Video shows short cilia in control embryo are not motile.

Supplementary movie 7

Supplemental movie 7. Video shows tether cilia in a gas11 morphant embryo are not motile.

Supplementary movie 8

Supplemental movie 8. Video shows tether cilia in a gas11 morphant embryo are not motile.

Supplementary movie 9a

Supplemental movie 9a. High speed video microscopy of tether cilia in a control embryo at 24 hpf showing vortices in the vicinity of the tether cilia and particle propelling along the growing otolith.

Supplementary movie 9b

Supplemental movie 9b. Same as 9a with particle tracking.

Supplementary movie 10a

Supplemental movie 10a. High speed video microscopy of the inner ear in a control embryo at 23 hpf showing high displacement of otolith precursors in the vicinity of tether cilia and low displacement away from tether cilia.

Supplementary movie 10b

Supplemental movie 10b. Same as supplemental movie 10a with particle tracking.

Supplementary movie 11a

Supplemental movie 11a. High speed video microscopy of tether cilia in a gas11 morphant at 25 hpf showing very low particle displacement in the vicinity of tether cilia.

Supplementary movie 11b

Supplemental movie 11b. Same as 11a with particle tracking.

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