FIGURE 3. Engraftment is initiated by asynchronous HSPC cell divisions.
From the following article:
Live-animal tracking of individual haematopoietic stem/progenitor cells in their niche
Cristina Lo Celso, Heather E. Fleming, Juwell W. Wu, Cher X. Zhao, Sam Miake-Lye, Joji Fujisaki, Daniel Côté, David W. Rowe, Charles P. Lin & David T. Scadden
Nature 457, 92-96(1 January 2009)
doi:10.1038/nature07434
a, HSPC progeny were imaged 1 day after injection in irradiated recipients (n = 4 mice), revealing heterogeneity in cell clustering. Blue, bone; red, vasculature; green, osteoblasts; white, HSPC progeny. b, Cells were tracked from day 0 to day 2 (n = 2 recipient mice) or from day 1 to day 2 (n = 3 recipient mice) and diverse kinetics of cell division were observed. c, Increasing numbers of clusters containing 2 or 
3 cells were observed in the days after injection (n = 4; error bars indicate s.e.m.). d, When 50% of cells were stained with DiD and 50% with DiI before injection, only single-colour clusters were observed. Red, DiD; green, autofluorescence. Arrows point at each DiD-positive cell within two clusters; arrowheads point at autofluorescent cells. Cells accepted for assessment had a dye/autofluorescence signal ratio >2 (in this example 2.82, 3.71, 4.12, 8.22). The same analysis was used to validate DiI signals. Scale bars in a, b, d are 50 
m. e, Summary of observed cell clusters in three independent experiments.
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