FIGURE 5. MFN2 regulates ER Ca²⁺ levels and its transfer to mitochondria.

a, Fura-2 recordings of cytosolic Ca²⁺ ([Ca²⁺]_i) after passive discharge of ER Ca²⁺ stores by tert-buthyl benzohydroquinone (tBuBHQ; 0.1 mM) in MEFs of the indicated genotype. b, MT-AEQ measurements of [Ca²⁺]_m in response to tert-buthyl benzohydroquinone (0.1 mM) in MEFs of the specified genotype. Where specified, MFN2 was co-transfected with ER-AEQ. c, Mean and s.e.m. (n = 5) of [Ca²⁺]_m from a. d, ER-AEQ measurements of [Ca²⁺]_ER in cells of the indicated genotype. Where specified, MFN2 was co-transfected with ER-AEQ. Stores were replenished by perfusing cells with high Ca²⁺ (1.5 mM) Krebs-Ringer buffer. e, MT-AEQ recordings of [Ca²⁺]_m in wild-type and Mfn2^-/- cells challenged with 0.2 mM or 10 M ATP, respectively. f, Expanded abscissa scale of e. g, Mean and s.e.m. (n = 5) of peak [Ca²⁺]_m from e. Asterisk, P < 0.05 in a two sample Student's t test. h, Mean and s.e.m. (n = 5) of [Ca²⁺]_m uptake rate from e. Double asterisks, P < 0.001 in a two sample Student's t test.

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