FIGURE 2. hGFAP-Cre+;p53lox/lox;Ptenlox/+ gliomas mirror key features of human malignant gliomas.
From the following article:
p53 and Pten control neural and glioma stem/progenitor cell renewal and differentiation
Hongwu Zheng, Haoqiang Ying, Haiyan Yan, Alec C. Kimmelman, David J. Hiller, An-Jou Chen, Samuel R. Perry, Giovanni Tonon, Gerald C. Chu, Zhihu Ding, Jayne M. Stommel, Katherine L. Dunn, Ruprecht Wiedemeyer, Mingjian J. You, Cameron Brennan, Y. Alan Wang, Keith L. Ligon, Wing H. Wong, Lynda Chin & Ronald A. DePinho
Nature 455, 1129-1133(23 October 2008)
doi:10.1038/nature07443
a, Pten expression is completely extinguished in tumour cells. Sections of three independent malignant gliomas were stained with haematoxylin and eosin (H&E) and an anti-Pten antibody. 'N' indicates the adjacent normal regions of the tumour cells; the arrows point to Pten-positive vascular cells embedded in the tumour. b, The wild-type Pten allele is lost in glioma cells. Genomic DNA isolated from liver tissues and brain tumour cells was subjected to PCR-based assays for genotyping Pten and p53 alleles. '+' designates the Pten wild-type allele, 'L' denotes the conditional allele, and 'D' denotes the inactivated form of the conditional allele after Cre-mediated recombination. c, Immunohistochemical staining of mouse normal brain or glioma sections with antibodies against activated phosphorylated Akt (pAkt), phosphorylated ribosomal protein S6 kinase (pS6) and Vegf. d, TNS lines isolated from independent malignant gliomas were cultured in NSC medium or differentiation medium (1% fetal bovine serum (FBS)) and immunostained for Nestin, Gfap and Tuj1 as indicated. Scale bars, 50 
m (a, c); original magnification in d 
400.
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