FIGURE 2. Sae2 is phosphorylated by Cdc28 on Ser 267.
From the following article:
CDK targets Sae2 to control DNA-end resection and homologous recombination
Pablo Huertas, Felipe Cortés-Ledesma, Alessandro A. Sartori, Andrés Aguilera & Stephen P. Jackson
Nature 455, 689-692(2 October 2008)
doi:10.1038/nature07215
a, TAP-tagged Sae2 derivatives were immunoprecipitated and detected as indicated. b, TAP-tagged Sae2 was purified from G1 or G2 arrested cultures. U, G2 arrested untagged control cells. Immunoprecipitated samples and inputs (5%) were immunoblotted as indicated. c, Glutathione S-transferase (GST)-fused Sae2 and Sae2-S267A were purified, incubated with recombinant Cdk2/Cyclin A and ATP, resolved by 10% SDS–PAGE and immunoblotted as indicated. d, Recombination frequencies of strains in a hairpin-containing recombination system22. e, Spores after 24 h in sporulation medium. f, Spore viability 24 h after the addition of sporulation medium (SPM)10. Error bars in d–f represent s.d. (n = 2).
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