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Article
Nature 454, 728-734 (7 August 2008) | doi:10.1038/nature07212; Received 13 March 2008; Accepted 25 June 2008; Published online 27 July 2008
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A mechanism for asymmetric segregation of age during yeast budding
Zhanna Shcheprova1, Sandro Baldi1, Stephanie Buvelot Frei1, Gaston Gonnet2 & Yves Barral1
- Institute of Biochemistry, Biology Department, ETH Zurich, Schafmattstrasse 18, 8093 Zurich, Switzerland
- Institute of Computational Science, Department of Computer Science, ETH Zurich, Universtätstrasse 6, 8092 Zürich, Switzerland
Correspondence to: Yves Barral1 Correspondence and requests for materials should be addressed to Y.B. (Email: yves.barral@bc.biol.ethz.ch).
Abstract
Ageing and the mortality that ensues are sustainable for the species only if age is reset in newborns. In budding yeast, buds are made young whereas ageing factors, such as carbonylated proteins and DNA circles, remain confined to the ageing mother cell. The mechanisms of this confinement and their relevance are poorly understood. Here we show that a septin-dependent, lateral diffusion barrier forms in the nuclear envelope and limits the translocation of pre-existing nuclear pores into the bud. The retention of DNA circles within the mother cell depends on the presence of the diffusion barrier and on the anchorage of the circles to pores mediated by the nuclear basket. In accordance with the diffusion barrier ensuring the asymmetric segregation of nuclear age-determinants, the barrier mutant bud6
fails to properly reset age in buds. Our data involve septin-dependent diffusion barriers in the confinement of ageing factors to one daughter cell during asymmetric cell division.
- Institute of Biochemistry, Biology Department, ETH Zurich, Schafmattstrasse 18, 8093 Zurich, Switzerland
- Institute of Computational Science, Department of Computer Science, ETH Zurich, Universtätstrasse 6, 8092 Zürich, Switzerland
Correspondence to: Yves Barral1 Correspondence and requests for materials should be addressed to Y.B. (Email: yves.barral@bc.biol.ethz.ch).
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