FIGURE 2. Tel1- and Sae2-mediated end processing suppresses mutagenic NHEJ and chromosome translocations.

ChIP assays were used to assess the levels of RPA at the DSB in dun1, h2a-S129A, sae2, sae2^2,5,6,8,9, tel1 and tel1-kd strains using an anti-RPA antibody as described in Methods. a, The positions of the HO cut site and the location of primers. b, QAOS assay that measures the amount of ssDNA at 1.6-kb centromere proximal (HO1) to a DSB site by primer extension at non-denaturing conditions using tagging primer HO1-ss, followed by real-time quantitative PCR using Tag and HO1-f primers²³. c, PCR signals from a primer set that anneals 0.2-kb distal to the HO break at different durations of HO expression were quantified and plotted as a graph. Fold immunoprecipitate represents the ratio of the RPA PCR signal before and after HO induction, normalized by the PCR signal of the PRE1 control. d, Percentage ssDNA was calculated by dividing the PCR signals using primer HO1-f and Tag with those from a denatured sample at each time point and plotted as a graph. Each point is the average  s.d. from two separate experiments.

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