FIGURE 3. Foxp3 interacts with ROR
t and inhibits RORt-directed IL-17 expression.
From the following article:
TGF-
-induced Foxp3 inhibits TH17 cell differentiation by antagonizing ROR
t function
Liang Zhou, Jared E. Lopes, Mark M. W. Chong, Ivaylo I. Ivanov, Roy Min, Gabriel D. Victora, Yuelei Shen, Jianguang Du, Yuri P. Rubtsov, Alexander Y. Rudensky, Steven F. Ziegler & Dan R. Littman
Nature 453, 236-240(8 May 2008)
doi:10.1038/nature06878
a, Co-immunoprecipitation of Foxp3 and RORt from extracts of co-transfected 293T cells with or without DNaseI or ethidium bromide (EtBr). Cells were transfected with mouse RORt and Flag-tagged wild-type (Flag–Foxp3) or exon 2-deleted (Flag–Foxp3
Ex2) Foxp3. Anti-Flag immunoprecipitates (IP) and total lysates were immunoblotted with anti-RORt antibody and anti-Flag antibody. b, Cells were transfected with Flag-tagged human RORT and full-length (FL) or the exon 2-deleted isoform of human FOXP3 (Ex2). Anti-Flag immunoprecipitates and total lysates were probed with anti-FOXP3 and anti-Flag antibodies. c, Naive CD4+ T cells were co-transduced with retroviruses encoding RORt (MIT vector, Thy1.1 reporter) and various mouse Foxp3 constructs (MIG vector, GFP reporter). IL-17 expression was assessed on day 4 in cells gated for expression of both Thy1.1 and GFP. Representative data from at least three experiments are shown in each of the panels.