FIGURE 2. Long-term multi-lineage reconstitution by HSCs and multipotent progenitors from p16^Ink4a-/-p19^Arf-/-Trp53^-/- mice.

a, Long-term reconstitution by double-sorted HSCs (CD150⁺Sca-1⁺c-kit⁺CD48^-Lin^-) from wild-type mice. b, Transplantation of HSCs from p16^Ink4a-/-p19^Arf-/-Trp53^-/- mice results in long-term multi-lineage reconstitution of recipient mice when analysed 14–20 weeks after transplantation (four of four mice). c, Double-sorted multipotent progenitors (Sca-1⁺c-kit⁺CD150^-CD48^-Lin^-) from wild-type mice are only capable of contributing to long-lived lymphocyte B220⁺ and CD3⁺ populations and not to short-lived myeloid Mac-1⁺ and Gr-1⁺ populations. d, Transplanted double-sorted multipotent progenitors from triple mutant mice also resulted in long-term multi-lineage reconstitution of recipient mice when analysed 14–20 weeks after transplantation (four of four mice). e, Double-sorted c-kit⁺Sca-1^-Lin^- myeloid progenitors do not contribute to short-lived myeloid Mac-1⁺ and Gr-1⁺ populations.